Midipreps for plasmid DNA purification—NucleoBond Xtra Midi/Midi Plus
NucleoBond Xtra Midiprep kits are a new generation of anion-exchange midiprep plasmid purification kits based on patented NucleoBond technology. Typical yields of ≥250 μg of ultra-pure plasmid DNA can be obtained in about half the time compared to other Midiprep kits. The entire plasmid purification procedure takes 68 minutes with NucleoBond Xtra Midi kits and 28 minutes with NucleoBond Xtra Midi Plus kits. Read the NucleoBond Xtra technote to see how it works.
NucleoBond Xtra Midiprep kits are a new generation of anion-exchange midiprep plasmid purification kits based on patented NucleoBond technology. Typical yields of ≥250 μg of ultra-pure plasmid DNA can be obtained in about half the time compared to other midiprep kits. The entire plasmid purification procedure takes 68 minutes with NucleoBond Xtra Midi kits and 28 minutes with NucleoBond Xtra Midi Plus kits. Read the NucleoBond Xtra technote to see how it works.
NucleoBond Xtra Midiprep kits contain an enlarged column with a lower silica resin bed. This in turn enables faster flow of lysate and buffers through the columns. Specially designed column filters are included for convenient and time-saving clarification of bacterial lysates. The column filters are supplied inserted in the NucleoBond Xtra midiprep columns and allow parallel clarification of bacterial lysate and loading onto the column. Their large, structured surface leads to high filter-flow rates and minimizes the risk of clogging. The improved silica material has greater DNA binding capacity and is based on the proven NucleoBond anion-exchanger group MAE (methylaminoethanol). Optimized buffer compositions additionally lead to improved alkaline lysis and increased column flow rates.
- Xtra fast—reduce prep time by up to 60%
- Xtra high yields—obtain up to 100% more transfection-grade DNA, typical yield: 250 µg
- Xtra purity—obtain the highest purity via proven anion-exchange technology
- Xtra flexible—choose from Midiprep/Maxiprep, with or without NucleoBond Finalizer (for ultra-fast purification)
|NucleoBond Xtra Midi||NucleoBond Xtra Midi Plus|
|Technology||Anion exchange||Anion exchange|
|Lysate clarification||Column filters||Column filters|
|Starting material||<200 ml (high-copy-number)
<400 ml (low-copy-number)
|<200 ml (high-copy-number)
<400 ml (low-copy-number)
|Vector size||<300 kb||<300 kb|
|Typical yield||400 µg||400 µg|
|Isopropanol precipitation||Centrifugation||NucleoBond Finalizer|
|Preparation time||68 min/4 preps||28 min/4 preps|
- Transfecting sensitive cells
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tip for a successful transfection—remove endotoxins!
Endotoxins are copurified during plasmid preparations from bacterial lysates and affect eukaryotic cell survival, ultimately leading to a lower transfection efficiency. Innovative mini-format technology reduces endotoxins in your plasmid prep, leads to more successful transfections, and simplifies your workflow.View the data Purchase info
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- NucleoBond Xtra Midi EF (Plus)/Xtra Maxi EF (Plus)
- NucleoSpin Tissue
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- NucleoSpin RNA/RNA Plus
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- NucleoZOL/NucleoSpin RNA Set for NucleoZOL
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