NucleoBond Xtra Combi Rack rack for use with NucleoBond Xtra Midi, Xtra Maxi, Xtra BAC, AX 100, AX 500, AX 2000, and AX 10000 Columns pack of 1

NucleoBond Xtra EF Buffer Set I contains the extra buffers needed for the isolation of endotoxin-free low-copy plasmids, BACs, PACs, and P1 constructs using NucleoBond Xtra Midi/Maxi EF kits. This set includes 150 mL of buffers RES-EF, LYS-EF, NEU-EF, and RNase A, and is sufficient for 12 Xtra Maxi preps or 18 Xtra Midi preps.

NucleoBond Xtra Buffer Set I buffer set for the isolation of low-copy plasmids, BACs, PACs, and P1 constructs, only applicable with NucleoBond Xtra kits, sufficient for 12 Xtra Maxi preps or 18 Xtra Midi preps - Buffer RES, Buffer LYS, Buffer NEU, RNase A

Endotoxins are copurified during plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. Macherey-Nagel has developed a new column format and a novel buffer chemistry to enable vacuum-processed isolation of transfection-grade plasmid DNA in a midiprep format.

Eluates from anion exchange plasmid preparations (such as NucleoBond midipreps and maxipreps) contain high salt concentrations. Macherey-Nagel has developed a new vacuum-driven accessory, the NucleoSnap Finisher Midi/Maxi kits, that allow you to desalt and concentrate plasmid DNA in a few minutes without any loss of yield or quality. These kits are the fastest way to desalt and concentrate DNA after your NucleoBond plasmid DNA preps. NucleoSnap Finisher Midi/Maxi kits supersede the commonly used and time-consuming isopropanol precipitation and centrifugation steps by allowing rapid DNA precipitation and concentration of large volumes under vacuum.

Cat. # 740434.50 includes sufficient quantities of reagents and materials for processing of 50 samples.