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Home › Products › Nucleic acid purification › Plasmid purification kits › Megaprep and gigaprep › NucleoBond PC 10000

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Plasmid gigaprep kits—NucleoBond PC 10000

Purify up to 10 mg of ultra-pure transfection grade plasmid DNA using MN's proven anion-exchange technology

NucleoBond PC 10000 plasmid gigaprep kits allow you to purify up to 9,000 µg of plasmid DNA in just a few hours without phenol/chloroform extraction. The kits can be used to purify high- and low-copy-number plasmids, cosmids, BACs, PACs, and YACs grown from bacterial cultures. The resulting plasmids are at least as pure as plasmids isolated by traditional cesium chloride procedures and can be used for the most demanding molecular biology applications, including mammalian transfection, in vitro transcription, and automated sequencing.

Cat. # Product Size License Quantity Details
740593 NucleoBond® PC 10000 5 Preps USD $505.00

NucleoBond PC 10000 (5) 5 preps for the isolation up to 9,000 µg of plasmid DNA - NucleoBond AX 10000 Columns, NucleoBond Folded Filters, buffers, RNase A

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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The NucleoBond PC plasmid purification protocol

The NucleoBond PC plasmid purification protocol
The NucleoBond PC plasmid purification protocol.

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Transfection of HeLa cells with NucleoBond-purified plasmid DNA

Transfection of HeLa cells with NucleoBond-purified plasmid DNA
Transfection of HeLa cells with NucleoBond-purified plasmid DNA. Visualization was performed with GFP (Green Fluorescent Protein). Data kindly provided by Dr. Niller, Mr. Vogel, Institute for Medical Microbiology and Hygiene, University of Regensburg, Germany.

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740593: NucleoBond PC 10000

740593: NucleoBond PC 10000

Midiprep plasmid purfication kits Midiprep plasmid kits
Nucleic acid purification kits Plasmid maxiprep
Transfection grade plasmid purifcation kits Transfection-grade plasmid kits

Overview

  • Isolate highly pure plasmid DNA for transfection or sequencing
  • Rapid purification procedure using gravity flow
  • Clear lysates without centrifugation (filters provided with the kit)
  • Highly reproducible purification results

More Information

Technology Anion exchange
Format Giga
Lysate clarification Folded filters
Starting material 500–10,000 ml (high-copy-number)
Vector size <300 kb
Typical yield 5,000–9,000 µg
A260/280 1.80–1.95
Preparation time 120–150 min/2 preps

Applications

  • Transfecting sensitive cells
  • Sequencing
  • Cloning
  • PCR
  • Restriction analysis

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Plasmid purification articles

Plasmid kit selection guide

Find the right plasmid purification kit for your application.

Miniprep-scale, transfection-grade prep

Our kit reduces endotoxins from bacterial lysates compared to standard plasmid miniprep kits.

Large-scale, transfection-grade prep

Get high yields of endotoxin-free, transfection-grade plasmid DNA with ultra-fast midi and maxi kits.

Vacuum-processed, transfection-grade prep

Isolate 250 μg of transfection-grade plasmid DNA in only 35 minutes.


Product demonstration

Learn how the NucleoBond PC 10000 kits can help in large-scale purification of up to 10 mg of ultrapure, endotoxin-free plasmid DNA.

Other nucleic acid purification product demos

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Product demonstration

Learn how the NucleoBond PC 10000 kits can help in large-scale purification of up to 10 mg of ultrapure, endotoxin-free plasmid DNA.

Other nucleic acid purification product demos

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  • Human ACE2 stable cell line
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  • Viral and host sequencing
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  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
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  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
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  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
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  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
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