NucleoSpin cfDNA Midi
NucleoSpin cfDNA Midi is designed for rapid preparation of highly pure cell-free DNA from up to 5 ml of blood plasma using vacuum filtration. The application of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during the process (e.g., disposal of collected column flowthrough). Combining NucleoSpin cfDNA Midi with the NucleoVac 96 Vacuum Manifold and the Starter Set Midi enables processing of up to 24 samples in parallel in ~90 minutes.
NucleoSpin cfDNA Midi is designed for rapid preparation of highly pure cell-free DNA from up to 5 ml of blood plasma using vacuum filtration. The application of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during the process (e.g., disposal of collected column flowthrough). Combining NucleoSpin cfDNA Midi with the NucleoVac 96 Vacuum Manifold and the Starter Set Midi enables processing of up to 24 samples in parallel in ~90 minutes.
The purification protocol is based on the lysis of the plasma samples using Proteinase K incubation in the presence of a chaotropic salt-containing buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded onto the binding columns. After loading and vacuum filtration-driven binding of the DNA to the silica membrane, impurities are removed by several washing steps. cfDNA is eluted after a final drying step.
Overview
- Superior recovery of fragmented cell-free DNA
- Parallel purification of 24 samples—special adapter set for NucleoVac 96 Vacuum Manifold
- Optimized protocol for Cell-Free DNA BCT (“Streck Tubes”)
More Information
| Technology | Silica membrane |
| Format | Midi spin columns |
| Starting material | 1–5 ml plasma |
| Blood draw tubes | EDTA, Cell-Free DNA BCT (Streck) |
| Fragment size | ≥50 bp |
| Typical yield | Varies depending on sample source, storage, and quality |
| Elution volume | 200 µl (140 µl final eluate volume) |
| Preparation time | 90 min/24 preps (EDTA plasma) |
Applications
- Typical downstream applications: NGS, qPCR, ddPCR
- Highly efficient recovery of cell-free DNA from liquid biopsies
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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