Magnetic bead-based nucleic acid purification from water and air samples—NucleoMag DNA/RNA Water
The NucleoMag DNA/RNA Water kit enables rapid manual or automated isolation of DNA and RNA from diverse water and air samples in a scalable, magnetic bead-based format. The kit is compatible with a variety of filters and filtration systems and can be used to process salty or fresh water samples ranging from turbid to clear. The kit protocol avoids the use of reducing agents while enabling efficient removal of inhibitors. Purified nucleic acids are suitable for downstream applications such as qPCR and NGS.
The NucleoMag DNA/RNA Water kit enables rapid manual or automated isolation of DNA and RNA from diverse water and air samples in a scalable, magnetic bead-based format. The kit is compatible with a variety of filters and filtration systems and can be used to process salty or fresh water samples ranging from turbid to clear. The kit protocol avoids the use of reducing agents while enabling efficient removal of inhibitors. Purified nucleic acids are suitable for downstream applications such as qPCR and NGS.
NucleoMag DNA/RNA Water provides a versatile, user-friendly solution for microbial metagenomics and environmental microbiology applications, and allows researchers to determine water quality or the efficiency of sterilization procedures by measuring the microbial load of a sample, including coliform, pathogenic, and free-living microorganisms. The kit includes magnetic beads and buffers that enable high-throughput processing of samples obtained using an array of filters and filtration systems (including 25-mm and 47-mm filters composed of various materials and cartridge filters such as Sterivex). Additional sample preparation steps—including RNase or DNase digestion or sample homogenization using MN Bead Tubes Type A—can be incorporated to optimize the protocol for particular sample types or downstream applications. In addition to enabling manual processing using magnetic separators, the automation-friendly format of NucleoMag DNA/RNA Water allows for the protocol to be easily adapted to popular platforms such as KingFisher and epMotion.
Overview
- Suitable for purification of microbial DNA and RNA from diverse salt and fresh water samples—ranging from turbid to clear—as well as from air filters
- Efficient removal of inhibitory compounds ensures optimal performance of downstream assays
- Compatible with a variety of filters and filtration systems
- Protocol enables incorporation of ceramic bead-based sample homogenization (using either size of MN Bead Tubes Type A) for optimal sample lysis
- Manual or automated processing performed at room temperature without reducing agents
More Information
| Technology | Magnetic-bead technology |
| Format | Highly reactive superparamagnetic beads |
| Processing | Manual or automated |
| Sample material | Water and air samples |
| Maximum amount of starting material | 10–1,000 ml per preparation |
| Fragment size | 300 bp–approx. 50 kb |
| Elution volume | 50–200 µl |
| Preparation time | 40 min on KingFisher Flex (excl. filtration and sample lysis) |
| Theoretical binding capacity | 0.4 µg/µl beads |
Applications
- Isolation of microbial DNA and RNA from filtered water and air samples
- Typical downstream applications: RT-PCR, qPCR, NGS
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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