Isolation of RNA from soil—NucleoBond RNA Soil and NucleoBond RNA Soil Mini
The NucleoBond RNA Soil and NucleoBond RNA Soil Mini kits are designed for the purification of RNA from soil and sediment samples. In combination with the DNA Set for NucleoBond RNA Soil (or DNA Set for NucleoBond RNA Soil Mini), RNA and DNA can be isolated from the same soil sample. Purified nucleic acids are suitable for metagenomic studies, for example, when the genome and transcriptome are analyzed from the same sample. NucleoBond RNA Soil Mini is a downscaled version of the NucleoBond RNA Soil kit.
The NucleoBond RNA Soil and NucleoBond RNA Soil Mini kits are designed for the purification of RNA from soil and sediment samples. In combination with the DNA Set for NucleoBond RNA Soil (or DNA Set for NucleoBond RNA Soil Mini), RNA and DNA can be isolated from the same soil sample. Purified nucleic acids are suitable for metagenomic studies, for example, when the genome and transcriptome are analyzed from the same sample. NucleoBond RNA Soil Mini is a downscaled version of the NucleoBond RNA Soil kit.
Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, these kits were designed with proven NucleoBond technology to enable variable sample inputs and yield pure nucleic acids. Sample lysis using our MN Bead Tubes Type A in the presence of the optimized Lysis Buffer E1 and phenol:chloroform:isoamyl alcohol ensures efficient sample disruption. The optional Buffer OPT enables efficient RNA and DNA purification from mineral and organic soil samples.
Overview
- Anion exchange technology to optimize RNA yield and purity
- Ideal for metagenomic studies
- Combination of mechanical homogenization and chemical lysis allows processing of large sample amounts
- Parallel preparation of RNA and DNA in one hour
More Information
| NucleoBond RNA Soil | NucleoBond RNA Soil Mini | |
| Technology | Anion-exchange chromatography | |
| Format | Gravity flow columns combined with MN Bead Tubes Type A | |
| Starting material | <2 g of soil | 0.25–0.5 g soil |
| Fragment size | ≥100 nt | |
| Typical yield | 1–10 µg | 0.25–2.5 µg |
| A260/A280 | 1.7–2.1 | 1.5–2.0 |
| A260/A230 | 1.8–2.5 | — |
| Typical RIN (RNA integrity number) | >8.5 | >7.0 |
| Elution volume | 100 µl | 50–100 µl |
| Preparation time | 60 min/6 preps | 60 min/12 preps |
| Binding capacity | 600 µg | 30 µg |
Applications
- RNA isolation from soil for metagenomic analysis
- Purified RNA is suitable for:
- Real-time RT-PCR
- Northern blotting
- Primer extension
- Array technology
- RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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