Isolation of genomic DNA from insects and crustaceans—NucleoSpin DNA Insect
The NucleoSpin DNA Insect kit is designed for the purification of genomic DNA from fresh, frozen, dried, or ethanol-preserved insect or crustacean samples. The isolated DNA can be used for genotyping, sequencing, or other common applications. The kit utilizes MN Bead Tubes Type D (steel beads; included in the kit) in combination with a common disruption device, such as an MN Bead Tube Holder or a swing mill, to lyse different cell types and tissues using a single procedure. MN bead tubes can also be ordered separately if needed.
Overview
- Suitable for any insect or crustacean sample—high-quality DNA from fresh, frozen, dried, or ethanol-preserved specimens
- Includes MN bead tubes for efficient lysis of the exoskeleton—compatible with most common disruption devices
- Fast and convenient procedure
More Information
| Technology | Silica-membrane technology combined with MN Bead Tubes Type D |
| Format | Mini spin columns |
| Starting material | <40 mg fresh, frozen, dried, or ethanol-preserved insect/crustacean sample |
| Fragment size | 200 bp–approximately 50 kb |
| Typical yield | <25 µg (varies by sample and disruption device) |
| A260/A280 |
1.7–1.9 |
| Elution volume | 25–200 µl |
| Preparation time | 35 min/6 preps |
| Binding capacity | 60 µg |
Applications
- Genomic DNA from fresh, frozen, dried or ethanol-preserved insect or crustacean samples
- Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
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