NucleoSpin 96 DNA RapidLyse
The isolation of genomic DNA (gDNA) from most organs is a challenging task. Organs are composed of different tissues with various types of cells and matrices. Large molecules such as collagens and proteoglycans can impede lysis and inhibit downstream enzymatic reactions such as PCR. The new NucleoSpin 96 DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency. This kit provides high-throughput DNA isolation for all common liquid handling platforms.
Overview
- Unique lysis chemistry efficiently releases gDNA from cells, tissues, and organs
- Manual or automated processing by vacuum, positive pressure, or centrifugation
- Easy automation on robotic platforms
More Information
| Technology | Silica membrane technology |
| Format | 96-well plates |
| Processing | Centrifuge, positive pressure, or vacuum (manual or automated) |
| Starting material |
Fresh, frozen, dried, or ethanol-preserved organs Eukaryotic cells Tail or ear clippings |
| Sample amount |
Up to 30 mg fresh weight, 1 x 106 cells |
| Lysis time | 60 min or less |
| Preparation time | 60 min/96 preps (excluding lysis) |
| Typical yield | Up to 4 µg of DNA per mg tissue |
| Elution volume | 100 µl |
| A260/280 |
1.7–1.9 |
| Binding capacity | 40 µg |
Applications
- Manual or automated isolation of DNA from human and animal tissue, cultured cells, or bacteria
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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