Genomic DNA extraction from plant tissue
NucleoSpin Plant II is a next-generation kit designed for the rapid isolation of genomic DNA from plant cells and tissue, providing higher yield and quality. The silica membrane in the NucleoSpin Plant II columns is optimized to improve DNA binding. Since plants are very heterogeneous and contain many different metabolites, such as polyphenols, polysaccharides, and acidic components, NucleoSpin Plant II offers two different lysis procedures for optimal processing of various samples.
NucleoSpin Plant II is a next-generation kit designed for the rapid isolation of genomic DNA from plant cells and tissue, providing higher yield and quality. The silica membrane in the NucleoSpin Plant II columns is optimized to improve DNA binding. Since plants are very heterogeneous and contain many different metabolites, such as polyphenols, polysaccharides, and acidic components, NucleoSpin Plant II offers two different lysis procedures for optimal processing of various samples. This kit includes two optimized, alternative lysis buffers based on the established CTAB and SDS lysis methods.
This kit allows you to process <100 mg of plant tissue (wet weight) or <20 mg of plant tissue (dry weight) with yields typically ranging from 1–30 µg of genomic DNA. The purified DNA is ready for use in subsequent reactions such as PCR, Southern blotting, and restriction analysis.
Medium- and large-scale genomic DNA isolation from plant tissue
The NucleoSpin plant midi and plant maxi kits are designed for isolating genomic DNA from medium to large samples of plant tissue. These kits also include two optimized, alternative lysis buffers, based on the established CTAB and SDS lysis methods, for optimal processing of various sample types.
Starting sample sizes range up to 250 mg (wet weight) for the plant midi kit and up to 1 g (wet weight) for the plant maxi kit. Typical yields range from 20 to 80 µg of DNA for NucleoSpin Plant Midi, and 60 to 260 µg of DNA for NucleoSpin Plant Maxi, depending on the size and source of the sample. The purified DNA is ready for use in subsequent reactions such as PCR, Southern blotting, and restriction analysis.
Overview
NucleoSpin Plant II
- Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
- Lysis Buffer PL1, based on CTAB lysis method
- Lysis Buffer PL2, based on SDS lysis method
- NucleoSpin Filters included for clarification of lysate
- Improved buffer compositions for higher yield and purity
- Optimized silica membrane for improved DNA binding
- RNase A included
NucleoSpin Plant Midi/Maxi
- Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
- Lysis Buffer C1, based on CTAB lysis method
- Lysis Buffer CL, based on SDS lysis method
- NucleoSpin Filters L included for clarification of lysate
- RNase A included
More Information
| NucleoSpin Plant II | NucleoSpin Plant Midi | NucleoSpin Plant Maxi | |
| Technology | Silica membrane | Silica membrane | Silica membrane |
| Format | Mini spin columns | Midi spin columns | Maxi spin columns |
| Starting material | <100 mg plant tissue (wet weight) <20 mg plant tissue (dry weight) |
<250 mg plant tissue (wet weight) |
<1,000 mg plant tissue (wet weight) |
| Lysate clarification | NucleoSpin Filters | NucleoSpin Plant L Filters |
NucleoSpin Plant XL Filters |
| Fragment size | 50 bp–30 kb | 50 bp–30 kb | 50 bp–30 kb |
| Typical yield | 1–30 µg | 20–80 µg | 60–260 µg |
| A260/280 | 1.6–1.9 | 1.8–1.9 | 1.8–1.9 |
| Elution volume | 2 x 50 µl | 200 µl | 500–2,000 µl |
| Preparation time | 30 min/prep | 75 min/prep | 90 min/prep |
Applications
Isolation of DNA from:
- Plant cells
- Plant tissue
- Fungi
Obtained DNA is suitable for:
- PCR
- Southern blotting
- Enzymatic reactions
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Greater yield and purity than plant DNA extraction kits from leading competitors
In this tech note we compare the yield and purity of genomic DNA recovered using NucleoSpin Plant II to DNA extracted using leading competitors. Data from a variety of difficult plant tissue samples are shown.
Read the tech note Plant RNA kitsTakara Bio USA, Inc.
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