Rapid and sensitive mycoplasma extraction—NucleoSpin Mycoplasma DNA

Successful extraction of even the smallest traces of mycoplasma DNA from cell culture material is possible with the highly sensitive NucleoSpin Mycoplasma DNA kit. Mycoplasma—a widespread and notoriously undetectable cell culture contaminant—is one of the greatest challenges for scientists performing cell culture. Mycoplasma contamination can significantly impact the integrity and reliability of cell-based assays and experiments, leading to unreliable research outcomes and potentially endangering the safety and efficacy of biopharmaceutical products.

With NucleoSpin Mycoplasma DNA, mycoplasmas are lysed quickly and efficiently by Lysis Buffer SML, which is a highly concentrated solution of chaotropic ions. Lysis buffer and ethanol create appropriate binding conditions to bind nucleic acids to the NucleoSpin Mycoplasma DNA Columns. Carrier RNA improves binding and recovery of low-concentrated nucleic acids. Contaminants (potential PCR inhibitors) like salts, metabolites, and soluble macromolecular cellular components are removed in simple wash steps with alcoholic buffers SMW1 and SMW2. Then, nucleic acids are eluted in water and are ready for use in subsequent reactions. Every kit lot is tested for nondetectability of mycoplasma DNA, ensuring no mycoplasma DNA background within the kit.

DNA extracted with the kit is suitable for qPCR-based mycoplasma DNA contamination testing. For a complete mycoplasma testing solution, we recommend pairing this kit with the TaKaRa Mycoplasma qPCR Detection Kit.