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  • ‹ Back to Plant and fungal RNA
  • NucleoSpin RNA Plant and Fungi
  • NucleoSpin RNA Plant
Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Isolate gDNA in parallel Adapt this kit to purify DNA and RNA in parallel
Home › Products › Nucleic acid purification › RNA purification kits › Plant and fungal RNA › NucleoSpin RNA Plant

RNA purification kits

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Use the dropdown list to select the nucleic acid purification kit for your application Nucleic acid purification product finder
Isolate gDNA in parallel Adapt this kit to purify DNA and RNA in parallel

Mini spin kit for isolation and purification of total RNA from plants—NucleoSpin RNA Plant

Purify total RNA from a broad range of plant tissues

NucleoSpin RNA Plant is designed for isolation and purification of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. The working procedure is similar to the standard RNA purification method without the use of organic compounds (e.g., Trizol). Removal of contaminating DNA is achieved by effective rDNase digestion during the purification process.

NucleoSpin RNA Plant is designed for isolation and purification of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. The working procedure is similar to the standard RNA purification method without the use of organic compounds (e.g., Trizol). Removal of contaminating DNA is achieved by effective rDNase digestion during the purification process.

The NucleoSpin RNA Plant kit features two alternative lysis buffers. In most cases, use of Buffer RA1 is recommended for lysis due to its strong denaturing properties. The presence of peculiar metabolites in a variety of plant tissues or fungi, however, may lead to solidification of the lysate, resulting in a non-processable slurry. In such cases, the alternative Buffer RAP is the buffer of choice. For thorough homogenization and reduction of lysate viscosity, NucleoSpin Filter Columns are also provided with the kit.

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Cat. # Product Size License Quantity Details
740949.50 NucleoSpin® RNA Plant 50 Preps USD $334.00

The NucleoSpin RNA Plant kit (50 preps) allows for the isolation of RNA from plant tissues without the use of harsh organic chemicals. The kit includes NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase-free rDNase.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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NucleoSpin RNA Plant procedure

NucleoSpin RNA Plant procedure
NucleoSpin RNA Plant procedure.

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740949.50: NucleoSpin RNA Plant

740949.50: NucleoSpin RNA Plant
740949.250 NucleoSpin® RNA Plant 250 Preps USD $1525.00

The NucleoSpin RNA Plant kit (250 preps) allows for the isolation of RNA from plant tissues without the use of harsh organic chemicals. The kit includes NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase-free rDNase.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin RNA Plant procedure

NucleoSpin RNA Plant procedure
NucleoSpin RNA Plant procedure.

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740949.250: NucleoSpin RNA Plant

740949.250: NucleoSpin RNA Plant
740949.10 NucleoSpin® RNA Plant 10 Preps USD $76.00

The NucleoSpin RNA Plant kit (10 preps) allows for the isolation of RNA from plant tissues without the use of harsh organic chemicals. The kit includes NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, and RNase-free rDNase.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

NucleoSpin RNA Plant procedure

NucleoSpin RNA Plant procedure
NucleoSpin RNA Plant procedure.

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740949.10: NucleoSpin RNA Plant

740949.10: NucleoSpin RNA Plant

Universal reagent for RNA isolation, no chloroform, no phase separation, easy procedure NucleoZOL reagent
Rapid isolation of genomic DNA from plant samples with high yield and quality Plant DNA purification
Selection guides RNA purification kit selection guide

Overview

  • Complete mini kit includes DNase I and shredders
  • No genomic DNA contamination
  • Optimal lysis conditions for all plant species—two alternative lysis buffers
  • No use of organic compounds (e.g., Trizol)
  • Yields up to 70 µg of high-quality, ready-to-use RNA
  • Simple spin-column protocol (no need to learn a new protocol)
  • Better performance and outstanding savings

More Information

Technology Silica membrane
Format Mini spin columns
Starting material 1–100 mg tissue
Fragment size 200 bp–20 kb
Typical yield 3–70 µg from 100 mg plant material
Elution volume 60 µl
Preparation time 30 min/6 preps

Applications

Isolate and purify total RNA from plant cells and tissue and filamentous fungi without the use of organic compounds (e.g., Trizol).

Purified RNA is suitable for:

  • Real-time RT-PCR
  • Northern blotting
  • Primer extension
  • Array technology
  • RNase protection assays

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


NucleoZOL RNA isolation reagent

Universal reagent for RNA isolation

NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials, including cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources.

See the data RNA purification kits

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  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
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  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
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  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
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