Mini spin kit for isolation and purification of total RNA from plants—NucleoSpin RNA Plant
NucleoSpin RNA Plant is designed for isolation and purification of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. The working procedure is similar to the standard RNA purification method without the use of organic compounds (e.g., Trizol). Removal of contaminating DNA is achieved by effective rDNase digestion during the purification process.
NucleoSpin RNA Plant is designed for isolation and purification of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. The working procedure is similar to the standard RNA purification method without the use of organic compounds (e.g., Trizol). Removal of contaminating DNA is achieved by effective rDNase digestion during the purification process.
The NucleoSpin RNA Plant kit features two alternative lysis buffers. In most cases, use of Buffer RA1 is recommended for lysis due to its strong denaturing properties. The presence of peculiar metabolites in a variety of plant tissues or fungi, however, may lead to solidification of the lysate, resulting in a non-processable slurry. In such cases, the alternative Buffer RAP is the buffer of choice. For thorough homogenization and reduction of lysate viscosity, NucleoSpin Filter Columns are also provided with the kit.
Overview
- Complete mini kit includes DNase I and shredders
- No genomic DNA contamination
- Optimal lysis conditions for all plant species—two alternative lysis buffers
- No use of organic compounds (e.g., Trizol)
- Yields up to 70 µg of high-quality, ready-to-use RNA
- Simple spin-column protocol (no need to learn a new protocol)
- Better performance and outstanding savings
More Information
| Technology | Silica membrane |
| Format | Mini spin columns |
| Starting material | 1–100 mg tissue |
| Fragment size | 200 bp–20 kb |
| Typical yield | 3–70 µg from 100 mg plant material |
| Elution volume | 60 µl |
| Preparation time | 30 min/6 preps |
Applications
Isolate and purify total RNA from plant cells and tissue and filamentous fungi without the use of organic compounds (e.g., Trizol).
Purified RNA is suitable for:
- Real-time RT-PCR
- Northern blotting
- Primer extension
- Array technology
- RNase protection assays
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Universal reagent for RNA isolation
NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials, including cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources.
See the data RNA purification kitsTakara Bio USA, Inc.
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