High-throughput RNA purification from plant and fungi—NucleoSpin 96 RNA Plant & Fungi

Obtaining high-quality RNA from plant and fungal samples, especially when you have a large number of samples, can be challenging and time-consuming. NucleoSpin 96 RNA Plant & Fungi is designed for quick and easy high-throughput isolation of RNA from diverse plant and fungal material, including samples rich in starch, sugar, secondary metabolites, and other compounds that might interfere with common RNA isolation procedures. The 96-well format has been tested successfully with a wide variety of plant and fungi samples, can be used with centrifugation or vacuum, and can be fully automated on common laboratory workstations.  

The protocol begins with mechanical disruption of your plant/fungal samples (using MN Bead Tubes or Bead Plates, grinding in liquid nitrogen, or any other suitable disruption method) in lysis buffer containing large amounts of chaotropic ions to effectively disrupt tough plant/fungal material. This lysis buffer also immediately inactivates RNases, which are present in virtually all biological materials. The NucleoSpin 96 Plant and Fungi Filter Plate quickly and efficiently removes plant/fungal debris, and a binding solution is added, which creates appropriate binding conditions that favor the adsorption of intact RNA to the silica membrane. Washing steps with two different specialized buffers remove salts, metabolites, and macromolecular cellular components. The resulting pure, high-quality RNA is eluted from the silica membrane, ready for downstream transcriptomic analysis.