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  • ‹ Back to Total RNA from cells and tissues
  • NucleoSpin RNA Plus
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Selection guides RNA purification kit selection guide
Magnetic-bead based isolation of RNA from tissue and cell samples NucleoMag RNA
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Home › Products › Nucleic acid purification › RNA purification kits › Total RNA from cells and tissues › NucleoSpin 96 RNA

RNA purification kits

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Selection guides RNA purification kit selection guide
Magnetic-bead based isolation of RNA from tissue and cell samples NucleoMag RNA
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High-throughput total RNA isolation & purification—NucleoSpin 96 RNA

Automated isolation and purification of total RNA from cell cultures and tissue in a 96-well plate format

NucleoSpin 96 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 96 RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit...

NucleoSpin 96 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 96 RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit. Ready-to-run protocols are available and can be customized to fit your needs. NucleoSpin 96 RNA provides consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination without the use of organic extraction (e.g., Trizol). These kits are powerful tools for applications such as microarray analysis in respect to gene expression profiling, and drug discovery.

The kits allow isolation and purification of total RNA from up to 107 cells or up to 30 mg of tissue (centrifuge processing). Contaminating DNA, which is also bound to the silica membrane, is removed by on-column DNA digestion using DNase I (DNase I is supplied with the kits). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is finally eluted under low ionic strength conditions with RNase-free water (pH 8.0–8.5).

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Cat. # Product Size License Quantity Details
740709.4 NucleoSpin® 96 RNA 4 x 96 Preps USD $1340.00

NucleoSpin 96 RNA (4 x 96) 4 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates MN Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, Round-well Block Low, buffers, RNase-free rDNase

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

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The NucleoSpin 8/96 RNA procedure

The NucleoSpin 8/96 RNA procedure
The NucleoSpin 8/96 RNA procedure.

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740709.4: NucleoSpin 96 RNA

740709.4: NucleoSpin 96 RNA
740709.24 NucleoSpin® 96 RNA 24 x 96 Preps USD $7215.00

NucleoSpin 96 RNA (24 x 96) 24 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates, MN Square-well Blocks, Round-well Block Low , Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNas

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoSpin 8/96 RNA procedure

The NucleoSpin 8/96 RNA procedure
The NucleoSpin 8/96 RNA procedure.

Back

740709.24: NucleoSpin 96 RNA

740709.24: NucleoSpin 96 RNA
740709.2 NucleoSpin® 96 RNA 2 x 96 Preps USD $760.00

NucleoSpin 96 RNA (2 x 96) 2 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates, MN Square-well Blocks, Round-well Block Low , Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoSpin 8/96 RNA procedure

The NucleoSpin 8/96 RNA procedure
The NucleoSpin 8/96 RNA procedure.

Back

740709.2: NucleoSpin 96 RNA

740709.2: NucleoSpin 96 RNA
740466.4 NucleoSpin® 96 RNA Core Kit 4 x 96 Preps USD $1150.00

NucleoSpin 96 RNA Core Kit (4 x 96) 4 x 96 preps for the isolation of total RNA from cells and tissue - NucleoSpin RNA Binding Plates, buffers, RNase-free rDNase

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoSpin 8/96 RNA procedure

The NucleoSpin 8/96 RNA procedure
The NucleoSpin 8/96 RNA procedure.

Back

740466.4: NucleoSpin 96 RNA Core Kit

740466.4: NucleoSpin 96 RNA Core Kit

automated nucleic acid purification Automate your workflow
Parallel processing of silica columns for nucleic acid purification by vacuum Vacuum manifolds
Efficient and quick RNA isolation from cells and tissues, including genomic DNA removal column NucleoSpin RNA Plus

Overview

  • High-throughput total RNA isolation and purification: 96-well plates
  • Fast and convenient sample processing with rDNase included in the kits
  • Reproducible yields for a wide variety of samples
  • Suitable for manual and automated processing, under vacuum or centrifugation
  • Innovative MN wash plate minimizes risk of cross-contamination
  • No organic extraction (e.g., Trizol) or precipitation

More Information

Technology Silica membrane
Format 96-well plates
Processing Manual or automated, vacuum or centrifugation
Starting material <107 cultured cells
30 mg tissue
Fragment size 200 bp–20 kb
Typical yield  <100 µg
Elution volume 50–130 µl
Preparation time 70 min/plate

Applications

Manual or automated isolation and purification of total RNA from cultured cells and tissue.

Application notes

The NucleoSpin 8/96 RNA kits are compatible with many major robotic systems. Application notes are available for:

  • Eppendorf epMotion 5075
  • CyBi-RoboSpense
  • X-Tractor Gene
  • AVISO Theonyx
  • Hamilton MICROLAB STAR
  • Tecan Freedom
  • Tecan Genesis / Te-Mo 96
  • MWG RoboSeq 4204


Additional product information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


RNA purification mini prep kits that stop RNA degradation and prevent genomic DNA contamination

The better way to isolate RNA

Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.

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