High-throughput total RNA isolation & purification—NucleoSpin 96 RNA

Automated isolation and purification of total RNA from cell cultures and tissue in a 96-well plate format

NucleoSpin 96 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 96 RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit. Ready-to-run protocols are available and can be customized to fit your needs. NucleoSpin 96 RNA provides consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination without the use of organic extraction (e.g., Trizol). These kits are powerful tools for applications such as microarray analysis in respect to gene expression profiling, and drug discovery.

The kits allow isolation and purification of total RNA from up to 10⁷ cells or up to 30 mg of tissue (centrifuge processing). Contaminating DNA, which is also bound to the silica membrane, is removed by on-column DNA digestion using DNase I (DNase I is supplied with the kits). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is finally eluted under low ionic strength conditions with RNase-free water (pH 8.0–8.5).

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Cat. #	Product	Size	Price
740709.4	NucleoSpin® 96 RNA	4 x 96 Preps	$1305.00
NucleoSpin 96 RNA (4 x 96) 4 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates MN Square-well Blocks, Elution Plates U-bottom, Self-adhering Foils, Round-well Block Low, buffers, RNase-free rDNase Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoSpin 8/96 RNA procedure The NucleoSpin 8/96 RNA procedure. Back 740709.4: NucleoSpin 96 RNA
740709.24	NucleoSpin® 96 RNA	24 x 96 Preps	$7030.00
NucleoSpin 96 RNA (24 x 96) 24 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates, MN Square-well Blocks, Round-well Block Low , Elution Plates U-bottom, Self-adhering Foils, buffers, RNase-free rDNas Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoSpin 8/96 RNA procedure The NucleoSpin 8/96 RNA procedure. Back 740709.24: NucleoSpin 96 RNA
740709.2	NucleoSpin® 96 RNA	2 x 96 Preps	$735.00
NucleoSpin 96 RNA (2 x 96) 2 x 96 preps for the isolation of RNA from cells and tissue - NucleoSpin RNA Binding Plates, MN Wash Plates, MN Square-well Blocks, Round-well Block Low , Elution Plates U-bottom, Self-adhering Foil, buffers, RNase-free rDNase Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoSpin 8/96 RNA procedure The NucleoSpin 8/96 RNA procedure. Back 740709.2: NucleoSpin 96 RNA
740466.4	NucleoSpin® 96 RNA Core Kit	4 x 96 Preps	$1115.00
NucleoSpin 96 RNA Core Kit (4 x 96) 4 x 96 preps for the isolation of total RNA from cells and tissue - NucleoSpin RNA Binding Plates, buffers, RNase-free rDNase Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents You May Also Like Image Data Back The NucleoSpin 8/96 RNA procedure The NucleoSpin 8/96 RNA procedure. Back 740466.4: NucleoSpin 96 RNA Core Kit

Overview

High-throughput total RNA isolation and purification: 96-well plates
Fast and convenient sample processing with rDNase included in the kits
Reproducible yields for a wide variety of samples
Suitable for manual and automated processing, under vacuum or centrifugation
Innovative MN wash plate minimizes risk of cross-contamination
No organic extraction (e.g., Trizol) or precipitation

More Information

Technology	Silica membrane
Format	96-well plates
Processing	Manual or automated, vacuum or centrifugation
Starting material	<10⁷ cultured cells 30 mg tissue
Fragment size	200 bp–20 kb
Typical yield	<100 µg
Elution volume	50–130 µl
Preparation time	70 min/plate

Applications

Manual or automated isolation and purification of total RNA from cultured cells and tissue.

Application notes

The NucleoSpin 8/96 RNA kits are compatible with many major robotic systems. Application notes are available for:

Eppendorf epMotion 5075
CyBi-RoboSpense
X-Tractor Gene
AVISO Theonyx
Hamilton MICROLAB STAR
Tecan Freedom
Tecan Genesis / Te-Mo 96
MWG RoboSeq 4204

Additional product information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

RNA purification mini prep kits that stop RNA degradation and prevent genomic DNA contamination

The better way to isolate RNA

Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.

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