High-throughput total RNA isolation from blood—NucleoSpin 8/96 RNA Blood

The NucleoSpin 8/96 RNA Blood kits combine the stringency of guanidine-isothiocyanate lysis with effective digestion of genomic DNA by rDNase treatment and the speed of silica membrane technology, allowing purification of total RNA from up to 400 µl of whole blood. These kits offer consistent RNA quality, reproducible yields of RNA, and high-throughput procedures free of cross-contamination.

In the NucleoSpin 8/96 RNA Blood procedure, blood cells are directly lysed by incubation in a solution containing large amounts of chaotropic ions. This lysis buffer immediately inactivates RNases and creates appropriate binding conditions which favor adsorption of RNA to the silica membrane of the provided binding strips/plates. Contaminating DNA, which is also bound to the silica membrane, is efficiently removed by on-column DNA digestion (a 15-min digestion at room temperature is sufficient, due to the highly specific activity of the rDNase). Salts, metabolites, and macromolecular cellular components are removed by simple washing steps with three different buffers. Pure RNA is eluted under low ionic strength conditions with RNase-free water.

The NucleoSpin 8/96 RNA Blood kits provide a powerful tool for diverse research applications, including gene expression profiling, drug discovery, and development of novel molecular diagnostics.