NucleoBond RNA/DNA—Isolation & purification of RNA & DNA from the same sample

Co-purification of large amounts of total RNA and a separate genomic DNA

NucleoBond RNA/DNA 80 and 400 kits are designed for the isolation and purification of up to 80 or 400 µg of ultra-pure total RNA, respectively, plus a separate genomic DNA fraction. A special manufacturing process guarantees RNase-free handling. These kits contain NucleoBond AXR columns (anion-exchange columns for the highest purity and yield) and all of the required buffers. Due to the resolving power of NucleoBond anion-exchange resin, contaminants are washed away, and RNA and genomic DNA can be eluted separately.

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Overview

Extra-high purity for up to 400 µg of total RNA via anion-exchange chromatography
Separate isolation of different RNA species (tRNA, rRNA, mRNA)
Separate elution of genomic DNA

More Information

	NucleoBond RNA/DNA 80	NucleoBond RNA/DNA 400
Technology	Anion-exchange chromatography	Anion-exchange chromatography
Format	Midi gravity-flow columns	Maxi gravity-flow columns
Starting material	5 x 10⁶ cultured eukaryotic cells 20 mg tissue 0.5 x 10⁸ bacteria/yeast cells	2 x 10⁷ cultured eukaryotic cells 100 mg tissue 2 x 10⁹bacteria/yeast cells
Fragment size	50 bp–300 kb	50 bp–300 kb
Typical yield	70 µg from 5 x 10⁶ cultured cells 30 µg from 20 mg tissue 50 µg from 0.5 x 10⁸ bacteria/yeast cells	300 µg from 2 x 10⁷ cultured cells 150 µg from 100 mg tissue 200 µg from 2 x 10⁹bacteria/yeast cells
A_260/280	1.80–1.95	1.80–1.95
Preparation time	1.5–2.5 hr	1.5–2.5 hr
Binding capacity	80 µg	400 µg

Applications

Isolate and purify total RNA and genomic DNA from cultured cells, tissue, and bacteria.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Purify DNA from small samples like microdissected tissue, small blood samples, or small amounts of cells.

Designed for extra small samples

Purify DNA from small samples such as microdissected tissue, small blood samples, or small amounts of cells. Based on reliable silica-membrane technology, NucleoSpin Tissue XS overcomes the limitations of conventional kits using an innovative new column design with a funnel-shaped ring.

Learn more All genomic DNA kits

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