NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials. These include cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources. Isolate RNA using a simple, one-phase extraction with NucleoZOL instead of an inconvenient two-phase chloroform extraction with a competitor “Zol”, and minimize the risk of sample loss and phenol carryover. The isolated RNA is ready for use in qRT-PCR, microarrays, RNase protection assays, poly A+ isolation, blotting, and other applications. NucleoZOL provides several advantages over a leading competitor “Zol” for rapid miRNA and total RNA isolation in terms of yield, quality, and purity.
The NucleoSpin RNA Set for NucleoZOL is a mini spin kit designed to combine NucleoZOL RNA extraction with NucleoSpin RNA purification. The kit contains silica membrane-based columns and the required buffers. After a sample is lysed in NucleoZOL (not provided in the mini spin kit), and contaminating DNA and proteins are pelleted, the supernatant is mixed with an optimized binding buffer for efficient binding of miRNA and large RNA to the silica membrane. A convenient bind-wash-elute procedure allows highly efficient isolation of pure RNA (miRNA and large RNA).
| NucleoZOL product overview | |
|---|---|
| Technology | One-phase extraction |
| Format | Reagent |
| Starting material | <1 x 107 cultured cells, bacteria, and yeast <100 mg human/animal/plant tissue <0.4 ml (viral) fluids |
| Fragment size | miRNA (10–200 nt), large RNA (>200 nt) |
| Typical yield (total RNA) | Liver: 6–8 µg/mg tissue Kidney, spleen: 3–4 µg/mg tissue Muscle, brain, lung: 0.5–1.5 µg/mg Cultured cells: 4–10 µg/106 cells |
| Typical yield (large RNA) | Liver: 5–7 µg/mg tissue Kidney, spleen: 3–4 µg/mg tissue Muscle, brain, lung: 0.5–1.5 µg/mg Cultured cells: 3–8 µg/106 cells |
| A260/A280 | 1.8–2.1 |
| Typical RIN (RNA Integrity Number) | >9 |
| Elution volume | Flexible |
| Preparation time | <1 hr |
| NucleoSpin RNA Set for NucleoZOL product overview | |
|---|---|
| Technology | Silica membrane technology |
| Format | Mini spin columns |
| Starting material |
≤500 µl NucleoZOL* sample |
| Fragment size | miRNA (10–200 nt), large RNA (>200 nt) |
| Typical recovery | 85–95% |
| Typical RIN (RNA Integrity Number) | Depending on sample quality (no significant loss of RIN detected) |
| Elution volume | 60 µl |
| Preparation time | <1 hr |
| Binding capacity | 200 µg |
*NucleoZOL not included.