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Tech Note

Comparison of NucleoZOL with a leading "Zol" competitor

NucleoZOL RNA isolation reagent for miRNA and total RNA

  • One-phase separation eliminates the need for chloroform
  • High yields of high-purity RNA from any starting material
  • miRNA and total RNA in separate fractions or a single fraction
  • Cells and tissues are lysed and homogenized directly in NucleoZOL reagent to eliminate any chance of RNA degradation
Product Summary Higher Yield Better RNA Quality Higher Purity Simpler Protocol

Product Summary  

NucleoZOL is a highly efficient RNA isolation reagent for extracting high-quality miRNA and total RNA from a variety of starting materials. These include cells, tissues, and liquids of human or animal origin, as well as plants, yeast, bacteria, viruses, and other sources. Isolate RNA using a simple, one-phase extraction with NucleoZOL instead of an inconvenient two-phase chloroform extraction with a competitor “Zol”, and minimize the risk of sample loss and phenol carryover. The isolated RNA is ready for use in qRT-PCR, microarrays, RNase protection assays, poly A+ isolation, blotting, and other applications. NucleoZOL provides several advantages over a leading competitor “Zol” for rapid miRNA and total RNA isolation in terms of yield, quality, and purity.

The NucleoSpin RNA Set for NucleoZOL is a mini spin kit designed to combine NucleoZOL RNA extraction with NucleoSpin RNA purification. The kit contains silica membrane-based columns and the required buffers. After a sample is lysed in NucleoZOL (not provided in the mini spin kit), and contaminating DNA and proteins are pelleted, the supernatant is mixed with an optimized binding buffer for efficient binding of miRNA and large RNA to the silica membrane. A convenient bind-wash-elute procedure allows highly efficient isolation of pure RNA (miRNA and large RNA).

NucleoZOL product overview
Technology One-phase extraction
Format Reagent
Starting material <1 x 107 cultured cells, bacteria, and yeast
<100 mg human/animal/plant tissue
<0.4 ml (viral) fluids
Fragment size miRNA (10–200 nt), large RNA (>200 nt)
Typical yield (total RNA) Liver: 6–8 µg/mg tissue
Kidney, spleen: 3–4 µg/mg tissue
Muscle, brain, lung: 0.5–1.5 µg/mg
Cultured cells: 4–10 µg/106 cells
Typical yield (large RNA) Liver: 5–7 µg/mg tissue
Kidney, spleen: 3–4 µg/mg tissue
Muscle, brain, lung: 0.5–1.5 µg/mg
Cultured cells: 3–8 µg/106 cells
A260/A280 1.8–2.1
Typical RIN (RNA Integrity Number) >9
Elution volume Flexible
Preparation time <1 hr
NucleoSpin RNA Set for NucleoZOL product overview
Technology Silica membrane technology
Format Mini spin columns
Starting material
≤500 µl NucleoZOL* sample
Fragment size miRNA (10–200 nt), large RNA (>200 nt)
Typical recovery 85–95%
Typical RIN (RNA Integrity Number) Depending on sample quality (no significant loss of RIN detected)
Elution volume 60 µl
Preparation time <1 hr
Binding capacity 200 µg

*NucleoZOL not included.

Higher Yield  

We compared NucleoZOL to the leading “Zol” two-phase extraction method. NucleoZOL resulted similar or greater yields with a far simpler protocol. RNA was extracted from a variety of starting materials and quantified using qRT-PCR.

Get similar or better RNA yields with NucleoZOL than a leading competitor “Zol”.

Better RNA Quality  

Total RNA was isolated using NucleoZOL from 60 mg mouse heart tissue and analyzed on an Agilent Bioanalyzer. Total RNA was isolated with NucleoZOL in combination with the NucleoSpin RNA Set for NucleoZOL (from 5 x 106 HeLa cells). RNA was analyzed on an Agilent Bioanalyzer; a RIN of 9 indicates high RNA quality.

Isolate high-quality RNA from fibrous tissue using NucleoZOL.

Higher Purity  

Total RNA was isolated from 60 mg rat lung tissue using NucleoZOL and a competitor “Zol” product. DNA contamination was quantified by qPCR (competitor “Zol”=100%). Compared to a standard two-phase extraction, only a minimal amount of DNA is carried over during purification with NucleoZOL (Panel A). The A260/A230 absorption ratio of total RNA isolated using NucleoZOL shows excellent purity, while the lower A260/A230 ratio of total RNA isolated using the competitor “Zol” indicates phenol carryover (Panel B).

Minimize DNA contamination and eliminate phenol carryover by using NucleoZOL instead of a leading competitor "Zol".

Simpler Protocol  

The NucleoZOL procedure is far easier than other “Zol” products because chloroform two-phase separation is not necessary. Cells and tissues are lysed and homogenized directly in NucleoZOL reagent, eliminating any chance of RNA degradation. Then contaminating molecules such as DNA, polysaccharides, and proteins are precipitated by the addition of water and removed by centrifugation. The NucleoZOL procedure allows the separate isolation of small and large RNA by adding ethanol and isopropanol, respectively. RNA can be reconstituted by RNase-free water.

NucleoZOL simplifies RNA isolation with a one-phase extraction

NucleoZol Competitor Zol
Sample homogenization Sample homogenization
Addition of water Addition of chloroform
Non-toxic Toxic
Centrifugation at RT Centrifugation at 4°C
Precipitation of contaminants Liquid-liquid phase separation
Aspiration of whole supernatant with RNA Aspiration of aqueous phase with RNA
Easy sampling of RNA Inconvenient pipetting for phase separation
Risk of carry-over of interphase/polar phase
Refrigerated centrifuge neccessary
Centrifugation at RT Centrifugation at 4°C
Precipitation of RNA Precipitation of RNA
Washing of RNA Washing of RNA
RNA in pellet RNA in pellet
Resuspension of RNA Drying and resuspension of RNA
No drying of RNA neccessary Time-consuming drying of RNA
Quick and easy

Advantages of NucleoZOL compared to a competitor “Zol”

NucleoZOL Common competitor products
Procedure One-phase procedure minimizes the risk of contamination by carryover Phase separation leads to risk of DNA / protein / phenol carryover or sample loss
Removal of DNA and proteins Precipitation of DNA and proteins results in minor risk of contamination Contamination possible due to difficult phase separation
Solubilization of RNA No drying required Time-consuming drying step required
miRNA isolation Protocol for fractionation of miRNA and total RNA No protocol for selective miRNA isolation available
Handling All steps are performed at room temperature Necessity of refrigerated centrifuge

Related Products

Cat. # Product Size License Quantity Details
740404.200 NucleoZOL™ 200 mL USD $321.00

NucleoZOL Reagent for the isolation of small and large RNA. (Bottle of 200 ml)

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents You May Also Like Image Data

Back

The NucleoZOL RNA isolation procedure

The NucleoZOL RNA isolation procedure
The NucleoZOL RNA isolation procedure.

Back

Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor

Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor
Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. DNA contamination was quantified by qPCR (competitor’s ZOL=100%). A minimal amount of DNA is carried over during purification with NucleoZOL, compared to the competitor’s ZOL.

Back

NucleoZOL provides similar or better RNA yields than a leading competitor

NucleoZOL provides similar or better RNA yields than a leading competitor
NucleoZOL provides similar or better RNA yields than a leading competitor. RNA was extracted from different starting materials using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. In each case, RNA was quantified by qRT-PCR and relative yields were calculated (NucleoZOL=100%). RNA isolation with NucleoZOL results in similar or better RNA yields than the competitor’s ZOL.

Back

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. The A260/A230 absorption ratio of total RNA isolated using NucleoZOL indicates excellent purity. The lower A260/A230 ratio of total RNA isolated using the competitor’s ZOL indicates a phenol carryover.

Back

740404.200: NucleoZOL

740404.200: NucleoZOL
740406.50 NucleoSpin® RNA Set for NucleoZOL™ 50 Preps USD $224.00

RNA purification with NucleoZOL* lysis and well-known NucleoSpin® RNA Columns. NucleoZOL is a highly efficient RNA isolation reagent for extracting total RNA and small RNA from a variety of sample types, using a one-phase extraction.

Package contains:
NucleoSpin® RNA Columns, Collection Tubes, buffers

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Image Data

Back

The NucleoSpin RNA Set for NucleoZOL procedure (after NucleoZOL lysis)

The NucleoSpin RNA Set for NucleoZOL procedure (after NucleoZOL lysis)
The NucleoSpin RNA Set for NucleoZOL procedure (after NucleoZOL lysis).

Back

High-quality total RNA was isolated from HeLa cells using NucleoZOL together with the NucleoSpin RNA Set for NucleoZOL

High-quality total RNA was isolated from HeLa cells using NucleoZOL together with the NucleoSpin RNA Set for NucleoZOL
High-quality total RNA was isolated from HeLa cells using NucleoZOL together with the NucleoSpin RNA Set for NucleoZOL. Total RNA was isolated with NucleoZOL in combination with the NucleoSpin RNA Set for NucleoZOL (from 5 x 106 HeLa cells). RNA was analyzed on an Agilent Bioanalyzer; a RIN of 9.8 indicates high RNA quality.

Back

NucleoZOL used together with the NucleoSpin RNA Set for NucleoZOL provided better total RNA yields than a leading competitor

NucleoZOL used together with the NucleoSpin RNA Set for NucleoZOL provided better total RNA yields than a leading competitor
NucleoZOL used together with the NucleoSpin RNA Set for NucleoZOL provided better total RNA yields than a leading competitor. Total RNA was isolated with NucleoZOL combined with mini spin columns (NucleoSpin RNA Set for NucleoZOL) or with a competitor product that combines a liquid extraction with a column-based method (Competitor Z). Total RNA yields were quantified using an Agilent 2100 Bioanalyzer.

Back

740406.50: NucleoSpin RNA Set for NucleoZOL

740406.50: NucleoSpin RNA Set for NucleoZOL

Required Products

Cat. # Product Size Price License Quantity Details
740404.200 NucleoZOL™ 200 mL USD $321.00

NucleoZOL Reagent for the isolation of small and large RNA. (Bottle of 200 ml)

Documents You May Also Like Image Data

Back

The NucleoZOL RNA isolation procedure

The NucleoZOL RNA isolation procedure
The NucleoZOL RNA isolation procedure.

Back

Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor

Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor
Total RNA isolated with NucleoZOL contains much less DNA contamination than a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. DNA contamination was quantified by qPCR (competitor’s ZOL=100%). A minimal amount of DNA is carried over during purification with NucleoZOL, compared to the competitor’s ZOL.

Back

NucleoZOL provides similar or better RNA yields than a leading competitor

NucleoZOL provides similar or better RNA yields than a leading competitor
NucleoZOL provides similar or better RNA yields than a leading competitor. RNA was extracted from different starting materials using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. In each case, RNA was quantified by qRT-PCR and relative yields were calculated (NucleoZOL=100%). RNA isolation with NucleoZOL results in similar or better RNA yields than the competitor’s ZOL.

Back

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor

No phenol carryover is observed when isolating total RNA with NucleoZOL, unlike a leading competitor. Total RNA was isolated from 50 mg rat lung tissue using NucleoZOL and a competitor’s ZOL (RNA isolation reagent) that requires a standard two-phase extraction. The A260/A230 absorption ratio of total RNA isolated using NucleoZOL indicates excellent purity. The lower A260/A230 ratio of total RNA isolated using the competitor’s ZOL indicates a phenol carryover.

Back

740404.200: NucleoZOL

740404.200: NucleoZOL

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