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Nucleic acid purification

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Product overview

Magnetic-bead-based isolation of viral RNA/DNA and bacterial DNA from fluids

The NucleoMag Pathogen kit is designed for the isolation of viral RNA/DNA and bacterial DNA from cell-free bodily fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes. The procedure is based on the reversible adsorption of nucleic acids to paramagnetic beads. Sample lysis is achieved by incubation with a lysis buffer containing chaotropic ions and supplemented by Proteinase K digestion. Nucleic acids are bound to the paramagnetic beads via addition of NucleoMag beads and binding buffer to the lysate. After magnetic separation with the NucleoMag Separator, the paramagnetic beads are washed with our specialized Wash Buffer to remove contaminants and salts.

  • Reagents and magnetic beads for isolation of 96 samples
  • One kit for all common clinical sample types
  • High sensitivity
  • Reliable nucleic acid isolation—suitable even for low viral titers

NucleoMag principle  

The NucleoMag family of products is based on magnetic bead technology. Nucleic acids are bound to the magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA and DNA are eluted from the beads using elution buffer. 

  NucleoMag bind           

Figure 1. NucleoMag magnetic bead technology. NucleoMag beads and binding buffer are added to the sample (left). Beads bound to DNA/RNA are held in place in the well by the magnet, and contaminants are washed away (middle). DNA/RNA is eluted while the beads are held in place by the magnet (right).

Procedure  

NucleoMag SEP

Figure 2. Overview of NucleoMag protocol for 96 samples.

Example data  

NucleoMag SEP ct value

Figure 3. A norovirus-positive stool sample and various dilutions of the sample were used for viral RNA purification using the NucleoMag Pathogen kit. Purified viral RNA was analyzed by real-time PCR.

Isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids,homogenized tissue sample suspensions, stool sample suspensions, and swab washes

RNA and DNA isolation from pathogens

The NucleoMag Pathogen kit is designed for the rapid manual and automated small-scale isolation of viral RNA and DNA and bacterial DNA from cell-free body fluids such as serum or plasma, blood, homogenized tissue sample suspensions, stool sample suspensions, and swab washes.

NucleoMag Pathogen NucleoMag SEP magnetic separator

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