| Technology | Silica-membrane technology |
| Format | Mini spin column |
| Sample material | <500 mg plant / fungal material |
| Fragment size | >200 nt |
| Typical yield | 20–70 µg |
| A260/A280 | 1.9–2.1 |
| A260/A230 | ~2 |
| Typical RIN (RNA integrity number) | 7–9 |
| Elution volume | 50 µl |
| Preparation time | 25 min / 6 preps |
| Binding capacity | 200 µg |
Product overview
NucleoSpin RNA Plant and Fungi
Plant and fungal material is diverse in terms of structure, cell wall composition, biochemical compounds and tissue type. Common products for RNA isolation from plant material usually work fine with samples such as Arabidopsis leaves but fail to efficiently purify RNA from more challenging samples. This kit was developed to efficiently purify high integrity RNA from samples rich in secondary metabolites that interfere with nucleic acid purification methods such as sugar, starch, or other compounds. NucleoSpin RNA Plant and Fungi enables the isolation of RNA from diverse plant tissues like seeds, roots, stems, and needles, as well as standard samples such as Arabidopsis leaves. The user manual includes tailored recommendations for successful sample preparation.
- Universal kit for challenging and standard plant and fungal samples
- Tailored protocols for optimal purification from diverse starting materials
- Includes filter columns for clearing lysate
- Typical downstream applications include qRT-PCR, Northern blotting, primer extension, arrays, and RNase protection assays
- MN Bead Tubes Type G are optional for homogenization in combination with a mixer mill (e.g., from Retsch).
At-a-glance
Protocol summary
First, plant material is mechanically disrupted (e.g. by MN bead tubes with a mixer mill, ground in liquid nitrogen, or any other suitable disruption method) in lysis buffer containing large amounts of chaotropic ions. The lysis buffer immediately inactivates RNases, which are present in virtually all biological materials. After removal of plant debris with the NucleoSpin Plant and Fungi Filter, a binding solution is added which creates appropriate binding conditions that favor adsorption of RNA to the silica membrane. Wash steps employing two separate buffers remove salts, metabolites, and macromolecular cellular components. Finally, pure RNA is eluted using RNase-free water.
Tested sample types
| Species | Material |
| Thale cress (Arabidopsis thaliana) | Leaves, seeds, siliques, roots |
| Alpine rock cress (Arabis alpina) | Leaves, stems |
| Tobacco (Nicotiniana tabacum) | Leaves |
| Wheat (Triticum aestivum) | Leaves |
| Barley (Hordeum vulgare) | Roots |
| Maize (Zea mays) | Grains, leaves |
| Fir (Abies procera) | Needles |
| Spruce (Picea abies) | Needles |
| Populus (Populus sp.) | Leaves, stems, roots |
| Potato (Solanum tuberosum) | Tuber |
| Grapevine (Vitis vinifera) | Leaves, grapes |
| Sugar beet (Beta vulgaris) | Roots |
| Sugar cane (Saccharum officinarum) | Stalks |
| Pea (Pisum sativum) | Roots |
| Alfalfa (Medicago sativa) | Roots |
| Clover (Medicago truncatula) | Roots, leaves |
| Banana (Musa sp.) | Pulp |
| Kiwi (Actinidia deliciosa) | Pulp |
| Citrus fruits (Citrus sp.) | Pulp |
| Blueberry (Vaccinium myrtillis) | Pulp |
| Apple (Malus domestica) | Pulp |
| Ginger (Zingiber officinale) | Rhizome |
| Mushroom (Laccaria bicolor) | Mycelium |
| Mushroom (Agaricus campestris) | Fruiting body |
| Grey mold (Botrytis cinerea) | Mycelium |
Data from plants
| Kit | MN | S | Q |
| Kiwi fruit | |||
| Yield [µg] | 7.0 | 2.3 | 0.8 |
| RIN | 8.0 | 2.1 | 3.8 |
| OD A260/A280 | 2.0 | 1.4 | 1.7 |
| Potato tuber | |||
| Yield [µg] | 1.9 | 1.1 | 1.3 |
| RIN | 8.0 | 8.2 | 5.0 |
| OD A260/A280 | 2.0 | 2.0 | 1.7 |
| Spruce needles | |||
| Yield [µg] | 57 | 12.1 | 1.1 |
| RIN | 7.2 | 4.2 | 5.6 |
| OD A260/A280 | 2.1 | 1.9 | 1.5 |
The NucleoSpin RNA Plant and Fungi kit enables efficient isolation of high integrity RNA from various sample types. After RNA isolation with the NucleoSpin RNA Plant and Fungi kit (MN) and two kits from competitors (S, Q), RNA was analyzed and quantified with an Agilent Bioanalyzer.
Data from fungi
The NucleoSpin RNA Plant and Fungi kit allows for extraction of high integrity RNA from fungal samples. The RNA was isolated from 3 independent samples of fungal fruiting body and fungal mycelium. Analysis of RNA was performed with an Agilent Bioanalyzer.
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Greater yield and purity than plant DNA extraction kits from leading competitors
In this tech note we compare the yield and purity of genomic DNA recovered using NucleoSpin Plant II to DNA extracted using leading competitors. Data from a variety of difficult plant tissue samples are shown.
Read the tech note Plant RNA kitsTakara Bio USA, Inc.
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