|Technology||Silica-membrane technology combined with MN Bead Tubes Type D|
|Format||Mini spin columns|
|Sample material||<40 mg fresh or frozen, lipid-rich tissue (e.g., brain, adipose tissue, fatty fish tissue)|
|Fragment size||200 bp–approx. 50 kbp|
|Typical yield||Depends on sample type, quality, and water content|
|Elution volume||25–200 µl|
|Preparation time||35 min/6 preps|
|Binding capacity||60 µg|
Genomic DNA purification
NucleoSpin DNA Lipid Tissue
The NucleoSpin DNA Lipid Tissue kit is designed for efficient isolation of genomic DNA from lipid-rich samples of human or animal origin. DNA can be isolated from a wide variety of fresh or frozen samples, such as brain, adipose tissue, and other tissue types that are rich in lipids, like fatty fish tissue.
It is often difficult to isolate DNA from lipid-rich tissue because the lipids either stop effective tissue disruption or affect the chemistry of the DNA isolation buffers. The NucleoSpin DNA Lipid Tissue kit combines enzymatic lysis and mechanical disruption of lipid-rich tissues with MN bead tubes.
- Special buffer composition for efficient removal of lipids
- MN bead tubes are included for efficient lysis
- Fast and convenient procedure without RNA contamination
- High DNA yield and purity compared to standard tissue kits
MN bead tubes
Prior to binding your sample to the miniprep binding columns the NucleoSpin DNA Lipid Tissue kit utilizes MN Bead Tubes Type D (steel beads; included in kit) in combination with a common disruption device, e.g., MN Bead Tube Holder (Cat. No. 740469, see below), or a swing mill to lyse different cell types and tissues within a single procedure. Additional MN bead tubes can be ordered separately (Cat. No. 740814.50) if needed.
DNA was isolated from three different lipid rich samples using the NucleoSpin DNA Lipid Tissue kit and compared to a standard tissue extraction kit. DNA was efficiently isolated with the NucleoSpin DNA Lipid Tissue kit even from difficult tissues like brain.
DNA was isolated from three different lipid rich samples using the NucleoSpin DNA Lipid Tissue kit (gray) and compared to a standard tissue extraction kit (blue) and the ratio of absorbance at 260 nm and 280 nm was calculated to assess purity of the isolated DNA. The optimal value of “1.8” is marked by a red line. DNA isolated with the NucleoSpin DNA Lipid Tissue kit was consistently pure.
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