Get SMARTer: tools and tips for RNA-seq

SMARTer NGS

The ability to study exceptionally rare or precious samples—including stem cells, circulating tumor cells, and brain tissue biopsies—requires extraordinary sensitivity and reproducibility. SMARTer RNA Sequencing (SMART-Seq) provides the tools you need to measure and understand the transcriptome complexity of extremely small samples.


See how an ideal dry-down pellet looks

Why? Drying your pellet sufficiently is important to maximizing cDNA recovery. However, over-drying the DNA pellet makes resuspension difficult.

How? There are visual clues that can help you determine when your pellet is dried just right.

Ideal bead pellet maximizes cDNA yield

 


Magnetic devices for SPRI bead purification

You may see references to building your own 0.2-ml tube magnetic separation device from rare earth bar magnets and a tip rack in some of our older user manuals, but we currently recommend the use of the SMARTer-Seq Magnetic Separator - PCR Strip, Cat. #635011.