Next-generation sequencing

• Product line overview
• Technical notes
  • Single-cell RNA- and DNA-seq
    • All-in-one cDNA synthesis and library prep from single cells
    • Highest sensitivity for single-cell mRNA-seq
    • Stranded libraries from single cells
    • Streamlined single-cell mRNA-seq
    • Full-length mRNA libraries from single cells (SMART-Seq v4)
    • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
    • Full-length mRNA libraries from single cells for Fluidigm C1 (SMART-Seq v4)
    • Full-length single-cell library method comparison
    • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
    • Next-gen WGA method for CNV and SNV detection from single cells
  • RNA-seq
    • Automation-friendly, all-in-one cDNA synthesis and library prep
    • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
    • Stranded libraries from picogram-input total RNA (v2)
    • Stranded libraries from FFPE inputs (v2)
    • Stranded libraries from 100 ng - 1 ug total RNA
    • Stranded libraries from 100 pg-100 ng total RNA
    • Stranded libraries from picogram-input total RNA (v1)
    • Stranded RNA-seq competitor kit comparison
    • Nonstranded libraries from FFPE inputs
    • Sensitive capture of full-length transcript information with targeted RNA-seq
  • DNA-seq
    • ThruPLEX HV outperforms NEBNext Ultra II
    • Accurate detection of low-frequency variants using molecular tags
    • Streamlined DNA-seq from challenging samples
    • Low cell number ChIP-seq using SMARTer ThruPLEX
    • Cell-free DNA sequencing
    • Sequencing analysis of low-frequency mutations in cfDNA
    • DNA-seq from FFPE samples
    • Low-input whole-exome sequencing
    • Tag-seq variant detection
    • Low-volume DNA shearing for SMARTer ThruPLEX library prep
  • Immune profiling
    • BCR repertoire profiling from human samples (bulk)
    • Improved TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from human samples (single cells)
    • TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from mouse samples (bulk)
    • BCR repertoire profiling from mouse samples (bulk)
  • Epigenetics and smRNA-seq
    • Full-length small RNA libraries
    • ChIP-seq libraries for transcription factor analysis
    • ChIP-seq libraries from ssDNA
    • Methylated DNA-seq
• Featured kits
• Technology and application overviews
  • SMART technology
  • Total RNA-seq
  • Biomarker discovery with RNA-seq
  • SMART-Seq PLUS solutions
  • Whole genome amplification from single cells
  • Cell-free nucleic acid sequencing
  • ThruPLEX HV data sheet
  • Sequencing depth for ThruPLEX Tag-seq
• FAQs and tips
  • RNA-seq FAQs
  • RNA-seq tips
  • Positive and negative controls in scRNA-seq
  • DNA-seq FAQs
  • ChIP-seq FAQs
  • Indexing FAQs
  • Cogent NGS Immune Profiler Software notices
• DNA-seq protocols
  • Using UMIs with ThruPLEX Tag-Seq HV
  • Exome capture with Illumina Nextera Rapid Capture
  • Targeted capture with Roche NimbleGen SeqCap EZ
  • Targeted capture with IDT xGen panels
  • Targeted capture with Agilent SureSelectXT
  • Targeted capture with Agilent SureSelectXT2
  • Targeted capture with Agilent SureSelectQXT
  • Plasma preparation for ThruPLEX Plasma-Seq workflow
• Webinars
  • Sign up: Total RNA sequencing of liquid biopsies
  • Sign-up: MeD-Seq, a novel method to detect DNA methylation
  • Sign up: automating full-length single-cell RNA-seq libraries
  • Sign up: single-cell DNA-seq webinar
  • Sign up: single-cell whole transcriptome analysis
  • Single-cell RNA-seq
  • Total RNA-seq
  • Immune profiling
  • Epigenetics
  • Targeted RNA-seq
  • DNA-seq
• Citations
  • Epigenetics citations
  • Microbiome citations
  • PicoPLEX citations
  • SMARTer RNA-seq citations
  • ThruPLEX DNA-seq citations
  • ThruPLEX Plasma-seq citations
• Posters