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Next-generation sequencing

Scientific posters

To educate and inform the scientific community about our cutting-edge NGS solutions, Takara Bio staff frequently presents scientific posters at major conferences around the world. Included below are scientific posters featuring methods and data for a wide array of SMARTer NGS applications.

Whole transcriptome analysis

Single-cell mRNA-seq

Pushing the limits of single-cell RNA-seq with SMART-Seq single cell technology (ABRF, March 2020) »

Robust and sensitive detection of gene fusions using high-throughput SMART-Seq chemistry on the ICELL8 cx system (AGBT, Feb 2020; ABRF, March 2020) »

SMART-Seq Stranded Kit performance with ovarian cancer cells (ABRF, March 2019) »

A SMARTer solution to stranded single-cell RNA-seq (ABRF, April 2018) »

SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System: improved chemistry for single cell transcriptome studies (NIH Single Cell Analysis Program Investigators Meeting, March 2016) »

Achieving unparalleled sensitivity and reproducibility in single-cell transcriptomics (ABRF, February 2016) »

An improved cDNA library generation protocol for transcriptome analysis from a single cell (ABRF, March 2014) »

Evaluation of commercially available RNA amplification kits at subnanogram input amounts of total RNA for RNA-seq (ABRF, March 2013) »

Total RNA-seq

SMART ligation-free tools for sequencing coding and non-coding RNA from low input samples (Keystone Symposia, February 2017) »

A complete solution for generating stranded RNA-seq libraries from high-input total RNA (ABRF, March 2015) »

SMART tools for strand-specific transcriptome analysis from mammalian total RNA (Keystone Symposia, March 2015) »

Strand-specific transcriptome sequencing for challenging samples (ABRF, March 2014) »

Development of a modified SMART system for robust transcriptome library preparation from limited quantities of compromised samples (ABRF, March 2013) »

High-throughput automation of single-cell mRNA-seq

Automation and miniaturization of low-input RNA-seq sample prep on the Mantis platform (SLAS, January 2016) »

Targeted sequencing

Immune profiling

Efficient high-throughput sequencing for quantitative immune profiling using unique molecular identifiers (ABRF, March 2020) »

High throughput single cell T-cell receptor profiling by SMART technology (AGBT, February 2018) »

A SMARTer approach to profiling the human T-cell receptor repertoire using the ICELL8 single-cell system (Royal Society of Chemistry, Single-Cell Genomics Meeting; September 2016) »

Single-cell T-cell receptor profiling with SMART technology (HudsonAlpha Immunogenomics Conference, September 2016) »

Profiling human TCR repertoire diversity with SMART technology (CAR-TCR Summit, September 2016) »

A SMARTer approach to profiling the human T-cell receptor repertoire (AAI, May 2016) »

A SMARTer approach to profiling the human T-cell receptor repertoire (ABRF, February 2016) »

Small RNA-seq

An unbiased and highly reproducible method for constructing microRNA NGS libraries for accurate expression profiling (ABRF, April 2018) »

Production of an unbiased, highly reproducible small RNA library for NGS using a novel circularization technology (ABRF, March 2017) »

Ligation-free small RNA sequencing libraries from clinical samples using SMART technology (Liquid Biopsies and Minimally-Invasive Diagnostics; September 2016) »

Targeted RNA-seq

A SMARTer approach to gene fusion identification (Next Generation Dx Summit 2017, August 2017) »

Sensitive capture of full-length transcript information with targeted RNA-seq (ASHG, October 2016) »

DNA sequencing

DNA-seq from cfDNA

ThruPLEX HV: a simplified system for preparation of molecular-tagged NGS libraries from FFPE and cell-free DNA (AMP, November 2019; AGBT, February 2020; ABRF, March 2020) »

Confident detection of low-frequency mutations in cell-free DNA using ThruPLEX technology with unique molecular tags (ABRF, April 2018) »

NGS libraries from cell-free DNA containing molecular tags prepared with ThruPLEX technology improve ability to detect rare alleles (ABRF, March 2017) »

ChIP-seq for low-input DNA

Harnessing template-switching technology for preparation of low-input ligation-free sequencing libraries (ABRF, March 2015) »

Harnessing template-switching technology for ChIP-seq applications (CSHL Epigenetics & Chromatin, September 2014) »

Single-cell DNA-seq

Next-generation whole genome amplification methods for CNV and SNV detection from single cells (Molecular Med Tri-Con, March 2019) »

PicoPLEX Gold: a new generation of single cell NGS library with high reproducibility, and greatly improved coverage and fidelity for precision medicine (AMP, October 2018) »

Sequencing single human cells and bacterial DNA using PicoPLEX DNA-seq at low coverage for aneuploidy, CNV, and genotyping applications (AGBT, February 2014) »

Whole exome sequencing

ThruPLEX as a high sensitivity library prep tool for whole exome and target panel sequencing (ASHG, September 2014) »

High-throughput automation of DNA-seq

Utilizing the Rheonix NGS OnePrep Solution to automate the Takara Bio ThruPLEX Tag-Seq HV library preparation kit (ABRF, March 2020) »

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