Next-generation sequencing

• Product line overview
• RNA-seq
  • Testimonials
  • Automated library prep
  • Technologies and applications
    • SMART technology
    • Single-cell mRNA-seq
    • Total RNA-seq
    • SMART-Seq PLUS solutions
  • Technotes
    • An easy protocol for total RNA-seq provides the widest input flexibility
    • Enabling long-read RNA sequencing from low-input samples
    • Singular for low input total RNA seq
    • All-in-one cDNA synthesis and library prep from single cells
    • Automation-friendly, all-in-one cDNA synthesis and library prep
    • All-in-one cDNA synthesis and library prep from ultra-low RNA inputs
    • 3' mRNA libraries from single cells (SMART-Seq v4 3' DE Kit)
    • Full-length mRNA-seq for target capture
    • Stranded libraries from single cells
    • Stranded libraries from picogram-input total RNA (v3)
    • Stranded libraries from 100 pg-100 ng total RNA
    • Stranded libraries from 100 ng - 1 ug total RNA
    • Stranded libraries from FFPE inputs (v2)
    • Nonstranded libraries from FFPE inputs
    • Singular and Takara Bio library prep
    • Full-length, single-cell, and ultra-low-input RNA-seq with UMIs
  • Webinars
    • Pushing the limits of sensitivity for single-cell applications
    • Capturing biological complexity by high-resolution single-cell genomics
    • Taking single-cell RNA-seq by STORM
    • STORM-seq Q&A
    • Neural multiomics Q&A
    • Liver metabolic function, dissecting one cell at a time
    • Pushing the limits Q&A
    • Total RNA sequencing of liquid biopsies
    • Liver metabolic function Q&A
    • Automating full-length single-cell RNA-seq libraries
    • Single-cell whole transcriptome analysis
    • Sensitivity and scale for neuron multiomics
  • RNA-seq tips
  • RNA-seq FAQs
• Technical notes
  • DNA-seq
    • Next-gen WGA method for CNV and SNV detection from single cells
    • Low-input whole-exome sequencing
    • DNA-seq from FFPE samples
    • Low cell number ChIP-seq using ThruPLEX DNA-Seq
    • Detection of low-frequency variants using ThruPLEX Tag-Seq FLEX
    • ThruPLEX FLEX outperforms NEBNext Ultra II
    • Streamlined DNA-seq from challenging samples
    • High-resolution CNV detection using PicoPLEX Gold DNA-Seq
    • ThruPLEX FLEX data sheet
    • Low-volume DNA shearing for ThruPLEX library prep
    • NGS library prep with enzymatic fragmentation
    • Comparing ThruPLEX FLEX EF to Kapa and NEBNext
  • Immune Profiling
    • Get started with BCR/TCR profiling
    • Track B-cell changes in your mouse model
    • Efficient and sensitive profiling of human B-cell receptor repertoire
    • TCRv2 kit validated for rhesus macaque samples
    • Improved TCR repertoire profiling from mouse samples (bulk)
    • TCR repertoire profiling from mouse samples (bulk)
    • BCR repertoire profiling from mouse samples (bulk)
    • Improved TCR repertoire profiling from human samples (bulk)
    • TCR repertoire profiling from human samples (single cells)
    • BCR repertoire profiling from human samples (bulk)
    • TCR and BCR immune profiling of human FFPE samples
  • Epigenetic sequencing
    • ChIP-seq libraries for transcription factor analysis
    • ChIP-seq libraries from ssDNA
    • Full-length small RNA libraries
    • Methylated DNA-seq with MBD2
  • Reproductive health technologies
    • Embgenix PGT-A DNBSEQ compatibility app note
    • Embgenix GT-omics for TE biopsies
    • Embgenix PGT-A
• Technology and application overviews
  • Embgenix GT-omics Oncology Tech Note
  • Sequencing depth for ThruPLEX Tag-seq
  • Whole genome amplification from single cells
• FAQs and tips
  • Positive and negative controls in scRNA-seq
  • DNA-seq FAQs
  • ChIP-seq FAQs
  • Indexing FAQs
  • TCR-seq methods: Q&A
• DNA-seq protocols
  • Using UMIs with ThruPLEX Tag-Seq FLEX
  • Targeted capture with Agilent SureSelectQXT
  • Exome capture with Illumina Nextera Rapid Capture
  • Targeted capture with Roche NimbleGen SeqCap EZ
  • Targeted capture with IDT xGen panels
  • Targeted capture with Agilent SureSelectXT
  • Targeted capture with Agilent SureSelectXT2
• Bioinformatics resources
  • Cogent NGS Analysis Pipeline
    • CogentAP FAQs
    • Cogent NGS Analysis Pipeline notices
  • Cogent NGS Discovery Software
    • Cogent NGS Discovery Software notices
  • Cogent NGS Immune Profiler
    • Cogent NGS Immune Profiler Software notices
  • Cogent NGS Immune Viewer
  • SMART-Seq DE3 Demultiplexer
• Webinars
  • Harnessing the power of full-length transcriptome analysis for biomarker discoveries
  • SMART-Seq Pro kits for biomarker detection
  • Takara Bio Single-Cell Workshop, Spring 2021
  • Single-Cell Workshop at 2020 NextGen Omics Series UK
  • Immunogenomics to accelerate immunotherapy
  • MeD-Seq, a novel method to detect DNA methylation
  • Single-cell DNA-seq
• Posters
  • Long-read mRNA-seq poster