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  • ‹ Back to Protein research
  • Capturem rapid purification technology
  • His-tag purification
  • Antibody purification
  • Phosphoprotein and glycoprotein purification
  • Other tag purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
    • Matchmaker Gold Yeast Two-Hybrid System
    • Make your own library for yeast two-hybrid screening
    • Mate and Plate yeast two-hybrid cDNA libraries
    • Aureobasidin A for improved selectable drug resistance in yeast
  • Expression systems
Yeast two-hybrid products Yeast two-hybrid products
Yeast culture media including YPD, YPDA, minimal media, and amino acid dropout mixes Yeast media
Quick and easy yeast transformation kit Quick & easy yeast transformation
Home › Learning centers › Protein research › Matchmaker Gold yeast two-hybrid systems

Protein research

  • Capturem rapid purification technology
    • FAQs about Capturem technology
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    • Rapid antibody labeling with Capturem technology
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  • His-tag purification
    • Why tag a protein?
    • Tech note: cobalt resin
    • Purification methods overview
    • Simplified purification of active, secreted his-tagged proteins
    • Overview: His60
    • Tech note: Capturem technology
    • Tech note: Capturem large volume
    • Magnetic beads
    • TALON resin selection guide
    • Selection guide: His60 resin
    • FAQs: TALON
    • Protocols
      • Video: Capturem his maxiprep
      • Video: Capturem his miniprep
      • Visual protocol: Capturem his maxiprep
      • Visual protocol: Capturem his miniprep
      • Capturem nickel column reagent compatibility
      • TALON reagent compatibility
      • His60 reagent compatibility
      • TALON: Native vs denaturing purification
      • Protocol: denaturing purification with TALON resin, pH elution
      • Protocol: native purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, pH elution
  • Antibody purification
    • Tech note: Rapid Protein G-based antibody purification
    • Tech note: thiophilic antibody purification resins
    • Tech note: Purification and screening
    • Tech note: IP and Co-IP
  • Phosphoprotein and glycoprotein purification
    • Non-tagged protein purification overview
    • Phosphoprotein purification overview
  • Other tag purification
    • Streptavidin-based enrichment using Capturem technology
    • Selection guide: peptide tags
    • Myc-tagged protein purification overview
    • GST-tagged protein purification overview
    • FLAG-tagged protein purification overview
  • Mass spectrometry digestion reagents
    • Protein and whole-cell lysate digestion with Capturem Trypsin technology
    • Protein digestion with Capturem Pepsin
  • Matchmaker Gold yeast two-hybrid systems
    • Matchmaker Gold Yeast Two-Hybrid System
    • Make your own library for yeast two-hybrid screening
    • Mate and Plate yeast two-hybrid cDNA libraries
    • Aureobasidin A for improved selectable drug resistance in yeast
  • Expression systems
    • Protein expression overview
    • Insect expression overview
    • Mammalian expression overview
    • pHEK293 Ultra expression overview
    • OKT3 expression in mammalian cells
    • Bacterial expression overview
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Yeast two-hybrid products Yeast two-hybrid products
Yeast culture media including YPD, YPDA, minimal media, and amino acid dropout mixes Yeast media
Quick and easy yeast transformation kit Quick & easy yeast transformation

Matchmaker Gold yeast two-hybrid systems

Matchmaker Gold systems are the most advanced and highest performing version of our highly popular Matchmaker product line for investigating protein-protein interactions and protein-DNA interactions. Features that make Matchmaker Gold systems unique are a combination of new yeast strains, stringent reporters, easy-to-use libraries, high-level expression vectors, and SMART-based library construction kits. We have tech notes demonstrating how these systems can be used for finding new protein interactions for your research projects.


Matchmaker Gold Yeast Two-Hybrid System

A novel reporter system that detects protein-protein interactions more easily, with fewer false positives.

Learn more

Make your own library for yeast two-hybrid screening

Learn how to make your own library for yeast two-hybrid screening.

Learn more

Mate and Plate yeast two-hybrid cDNA libraries

Learn how our Mate & Plate yeast two-hybrid libraries can simplify your Y2H screens.

Learn more

Aureobasidin A: selectable drug resistance for yeast

Learn more about how to use aureobasidin A to optimize your yeast two-hybrid screens.

Learn more

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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Capturem Trypsin for a rapid, efficient mass spectometry workflow at room temperature.

Speed up your mass spec workflow

Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

Capturem trypsin technology

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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