Insect expression overview

We offer a complete system and a variety of accessory tools for protein expression in insect host cells:

Baculovirus expression systems

Baculovirus expression systems are widely used to produce large quantities of recombinant proteins in insect host cells. These systems are particularly well-suited for proteins that are difficult to express in bacteria due to size, complexity, or posttranslational processing requirements. The posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles that of proteins expressed in mammalian cells rather than bacterial cells, so these proteins are more likely to have biological activities and immunological reactivities comparable to proteins that naturally exist in mammalian cells.

Baculovirus expression system & accessories
	Advantages	Applications
Baculovirus systems
BacPAK Baculovirus Expression System	High-yield protein expression (up to 1 g/L) Easy-to-use insect cells Posttranslational processing, biological activities, and immunological reactivities similar to mammalian systems His-tagged recombinant proteins that can be purified with TALON or His60 Ni resins	Expression of: Large and/or complex proteins Secreted proteins Proteins containing posttranslational modifications Multiprotein complexes
Baculovirus titration kits
BacPAK Baculovirus Rapid Titer Kit	Baculovirus titration within 48 hours via immunostaining of infected cells Very high signal-to-noise ratios Infection of target cells at known MOIs for precise and consistent results	Titration of any AcMNPV-type baculovirus, in order to optimize recombinant protein expression
BacPAK qPCR Titration Kit	Baculovirus titration in <4 hours via qPCR and TB Green detection Harvest, titration, and infection in a single day Infection of target cells at known MOIs for precise and consistent results	Titration of any AcMNPV-type baculovirus, in order to optimize recombinant protein expression
Baculovirus host cells and media
IPLB-Sf21 Insect Cells	Easy to use Compatible with the BacPAK Baculovirus Expression System and other baculoviral systems	Propagation of the AcMNPV baculovirus and its expression vector derivatives generated from the BacPAK Baculovirus Expression System

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