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  • Protein expression overview
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Protein research learning center
Baculovirus expression Baculovirus expression
Home › Learning centers › Protein research › Expression systems › Insect expression overview

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Protein research learning center
Baculovirus expression Baculovirus expression

Insect expression overview

We offer a complete system and a variety of accessory tools for protein expression in insect host cells:

Baculovirus expression systems

Baculovirus expression systems are widely used to produce large quantities of recombinant proteins in insect host cells. These systems are particularly well-suited for proteins that are difficult to express in bacteria due to size, complexity, or posttranslational processing requirements. The posttranslational processing and folding of recombinant proteins produced in insect cells more closely resembles that of proteins expressed in mammalian cells rather than bacterial cells, so these proteins are more likely to have biological activities and immunological reactivities comparable to proteins that naturally exist in mammalian cells.

Baculovirus expression system & accessories
 AdvantagesApplications
Baculovirus systems
BacPAK Baculovirus Expression System
  • High-yield protein expression (up to 1 g/L)
  • Easy-to-use insect cells
  • Posttranslational processing, biological activities, and immunological reactivities similar to mammalian systems
  • His-tagged recombinant proteins that can be purified with TALON or His60 Ni resins
Expression of:

  • Large and/or complex proteins
  • Secreted proteins
  • Proteins containing posttranslational modifications
  • Multiprotein complexes
Baculovirus titration kits
BacPAK Baculovirus Rapid Titer Kit
  • Baculovirus titration within 48 hours via immunostaining of infected cells
  • Very high signal-to-noise ratios
  • Infection of target cells at known MOIs for precise and consistent results
Titration of any AcMNPV-type baculovirus, in order to optimize recombinant protein expression
BacPAK qPCR Titration Kit
  • Baculovirus titration in <4 hours via qPCR and TB Green detection
  • Harvest, titration, and infection in a single day
  • Infection of target cells at known MOIs for precise and consistent results
Titration of any AcMNPV-type baculovirus, in order to optimize recombinant protein expression
Baculovirus host cells and media
IPLB-Sf21 Insect Cells
  • Easy to use
  • Compatible with the BacPAK Baculovirus Expression System and other baculoviral systems
Propagation of the AcMNPV baculovirus and its expression vector derivatives generated from the BacPAK Baculovirus Expression System

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Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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