Baculovirus titration kits
Accurate baculovirus titering is a vital step in determining the multiplicity of infection (MOI), a critical parameter for achieving optimal and consistent protein yield with baculovirus gene expression. However, traditional methods, such as plaque assays and end-point dilution assays, are laborious and take several days.
Our four BacPAK titration solutions are simple, accurate, and less time-consuming than traditional methods. They detect expression of either the baculoviral gp64 protein or p35 protein for quantification. The gp64 protein is expressed during the later stages of baculoviral infection and is only present in viruses capable of infection, while the p35 protein is expressed during the early stages of infection and can be correlated to functional titer.
Trying to decide which baculovirus titration product is right for your workflow? Check out the comparison chart below.
Overview
Our BacPAK titration solutions allow you to:
- Shorten baculovirus expression experiments by up to six days
- Eliminate troublesome plaque assays and end-point dilution assays
- Use with any AcMNPV-based baculovirus expression systems
- Harvest, titer, and infect in a single day
| BacPAK product comparison chart | ||||
|---|---|---|---|---|
| BacPAK Baculovirus GoStix Plus | BacPAK p35 ELISA Kit (Single Wash) | BacPAK Baculovirus Rapid Titer Kit | BacPAK qPCR Titration Kit | |
| Method | Lateral flow assay | ELISA | Immunological staining | qPCR |
| Assay time | 10 min | 20 hr | 2 days | 4 hr |
| Titers are based on | gp64 expression | p35 expression | gp64 expression | Viral genome content |
| Sensitivity | Low | Medium | Medium | High |
| Dynamic range | 1–30 ng gp64 | 1 x 105–1 x 107 PFU/ml | 1 x 105–1 x 107 PFU/ml | 1 x 104–1 x 108 PFU/ml |
| Advantages/disadvantages | Fastest and easiest method; provides accurate physical titer quantification but is low throughput. | Yields functional titer and can perform up to 96 samples at one time. Requires an overnight incubation, but little actual hands-on time. | Yields functional titer; can perform 96 samples at one time. | Potentially more sensitive than other methods, but does not give a readout of functional titer |
| When to choose this product | You want to quickly determine if your baculovirus is ready to harvest and/or you are willing to process samples individually. | You want to determine functional titer and want to process multiple samples at once. | You want to base your titers on gp64 measurement and want to process several samples at once. | You are most comfortable basing titers on viral genome content. |
More Information
Applications
- Determination of baculovirus titers to optimize recombinant protein expression
- AAV production
- Protein vaccine production
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
BacPAK p35 ELISA Kit
Learn how to obtain a functional baculovirus titer in <20 hr with the BacPAK p35 ELISA Kit
BacPAK Baculovirus GoStix Plus
Watch the BacPAK Baculovirus GoStix Plus in action to easily titer your baculovirus
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