mRNA interferase-MazF enzyme
MazF enzyme is a toxin protein in the toxin-antitoxin module of E. coli. It possesses endoribonuclease activity and specifically cleaves single-stranded RNA (ssRNA) at 5' ACA sequences. MazF does not cleave double-stranded RNA (dsRNA), double-stranded DNA, or single-stranded DNA. The mRNA interferase-MazF enzyme is supplied as a fusion protein of E. coli MazF and Trigger Factor, which is an E. coli chaperone protein. The enzyme is also supplied with 5X MazF Buffer consisting of 200 mM sodium phosphate (pH 7.5) and 0.05% Tween-20.
- Use for site-dependent cleavage of ssRNA
Provided as a solution of 20 mM sodium phosphate (pH 6.0), 0.01% Tween-20 and 50% glycerol.
Suzuki, M., Zhang, J., Liu, M., Woychik, N. A. & Inouye, M. Single Protein Production in Living Cells Facilitated by an mRNA Interferase. Mol. Cell 18 (2005).
Zhang, Y. et al. MazF Cleaves Cellular mRNAs Specifically at ACA to Block Protein Synthesis in Escherichia coli. Mol. Cell 12, 913–923 (2003).
Zhang, Y., Zhang, J., Hara, H., Kato, I. & Inouye, M. Insights into the mRNA cleavage mechanism by MazF, an mRNA interferase. J. Biol. Chem. 280, 3143–50 (2005).
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