We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to Matchmaker Gold yeast two-hybrid systems
  • Matchmaker Gold Yeast Two-Hybrid System
  • Make your own library for yeast two-hybrid screening
  • Mate and Plate yeast two-hybrid cDNA libraries
  • Aureobasidin A for improved selectable drug resistance in yeast
Learn more about our MatchMaker Gold Y2H systems. Matchmaker Gold yeast two-hybrid systems
Yeast two-hybrid products Yeast two-hybrid products
Yeast culture media including YPD, YPDA, minimal media, and amino acid dropout mixes Yeast media
Quick and easy yeast transformation kit Quick & easy yeast transformation
Home › Learning centers › Protein research › Matchmaker Gold yeast two-hybrid systems › Aureobasidin A for improved selectable drug resistance in yeast

Protein research

  • Capturem technology
    • Capturem protocols
    • Capturem tech notes and applications
    • FAQs about Capturem technology
    • Capturem technology citations
    • Capturem posters
  • Antibody purification and immunoprecipitation
    • IP and Co-IP of cardiac voltage-gated ion channel proteins
    • Tech note: Rapid Protein G-based antibody purification
    • Tech note: thiophilic antibody purification resins
    • Antibody purification and screening with Capturem Protein A
    • Tech note: IP and Co-IP
    • Rapid antibody labeling with Capturem technology
  • His-tag purification
    • Purification methods overview
    • TALON resin selection guide
    • Selection guide: His60 resin
    • xTractor Buffer is optimized for superior protein yield
    • Why tag a protein?
    • Tech note: cobalt resin
    • Simplified purification of active, secreted his-tagged proteins
    • Overview: His60
    • Tech note: Capturem technology
    • Tech note: Capturem large volume
    • Magnetic beads
    • FAQs: TALON
    • Protocols
      • Video: Capturem his maxiprep
      • Video: Capturem his miniprep
      • Visual protocol: Capturem his maxiprep
      • Visual protocol: Capturem his miniprep
      • Capturem nickel column reagent compatibility
      • TALON reagent compatibility
      • His60 reagent compatibility
      • TALON: Native vs denaturing purification
      • Protocol: denaturing purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, pH elution
  • Other tag purification
    • Streptavidin-based enrichment using Capturem technology
    • Selection guide: peptide tags
    • Myc-tagged protein purification overview
    • GST-tagged protein purification overview
  • Phosphoprotein and glycoprotein purification
    • Non-tagged protein purification overview
    • Phosphoprotein purification overview
  • Mass spectrometry digestion reagents
    • Capturem technology for trypsin protein digestion
    • Protein and whole-cell lysate digestion with Capturem Trypsin technology
    • Protein digestion with Capturem Pepsin
  • Matchmaker Gold yeast two-hybrid systems
    • Matchmaker Gold Yeast Two-Hybrid System
    • Make your own library for yeast two-hybrid screening
    • Mate and Plate yeast two-hybrid cDNA libraries
    • Aureobasidin A for improved selectable drug resistance in yeast
  • Expression systems
    • Protein expression overview
    • Insect expression overview
    • Mammalian expression overview
    • pHEK293 Ultra expression overview
    • OKT3 expression in mammalian cells
    • Bacterial expression overview
New products
Need help?
Contact Sales
Learn more about our MatchMaker Gold Y2H systems. Matchmaker Gold yeast two-hybrid systems
Yeast two-hybrid products Yeast two-hybrid products
Yeast culture media including YPD, YPDA, minimal media, and amino acid dropout mixes Yeast media
Quick and easy yeast transformation kit Quick & easy yeast transformation
Tech Note

Aureobasidin A: selectable drug resistance for yeast

  • Aureobasidin A resistance is a definitive selectable marker for yeast
  • Effectively reduces background in Matchmaker Gold yeast two-hybrid screens
  • Identifies genuine positives more easily, with fewer false positives
Introduction Low background with AbA screening AbA: ampicillin for yeast References

Introduction  

Aureobasidin A (AbA) is a potent and unique yeast antibiotic that kills S. cerevisiae at low concentrations (Takesako et. al. 1993). The drug is a cyclic multipeptide (Figure 1) that acts by inhibiting inositol phosphorylceramide synthase, an essential yeast enzyme. A mutant enzyme, encoded by the AUR1-C gene, confers resistance to AbA and can be used as a highly effective selectable marker that requires little to no optimization. Our optimized Matchmaker Gold Yeast Two-Hybrid System leverages this by including a reporter strain containing a stably-integrated AUR1-C gene, allowing for simple and rapid colony screening in yeast two-hybrid (Y2H) libraries using AbA selection.

Structure of Aureobasidin A

Figure 1. Structure of Aureobasidin A. Aureobasidin A (AbA; MW 1,100) is a cyclic depsipeptide antibiotic isolated from the fungus Aureobasidium pullulans R106. AbA inhibits the product of the yeast AUR1 gene (inositol phosphorylceramide synthase) and is toxic to S. cerevisiae at low concentrations (0.1 µg/ml). The gene product of a dominant mutant allele, AUR1-C, confers resistance to AbA and its expression can be used as a selectable marker.

Low background with AbA screening  

AbA selection virtually eliminates the high number of background colonies that often plague low-stringency primary screens that rely on nutritional markers alone (e.g., HIS3). Because AbA actually kills sensitive cells, rather than merely retarding their growth, AbA-based selection greatly favors the growth and identification of true positive clones. In general practice, a high percentage of clones that emerge from low-stringency primary screens using AbA selection are subsequently verified on high-stringency secondary screens that select for all four Matchmaker Gold reporters (AUR1-C, HIS3, ADE2, and MEL1).

AbA: ampicillin for yeast  

Many researchers have yearned for a yeast selection system similar to those used for E. coli or mammalian cells. In fact, AbA is used for yeast in a similar way as ampicillin and kanamycin are used for cloning in E. coli, or as G418 is used for selection of stably-transfected mammalian cell clones. AbA resistance is far easier to use in Y2H library screening than auxotrophic reporters, which often require additional optimization steps to achieve selective growth conditions.

If you wish to take your search for protein-protein interactions to the next level, look no further than AbA selection with the Matchmaker Gold Yeast Two-Hybrid System.

References  

Takesako, K. et al. Biological properties of aureobasidin A, a cyclic depsipeptide antifungal antibiotic. J. Antibiot. (Tokyo). 46, 1414–20 (1993).

Related Products

Cat. # Product Size License Quantity Details
630490 Make Your Own "Mate & Plate™" Library System 5 Rxns USD $1528.00

Mate & Plate Libraries are by far the easiest libraries to screen for protein-protein interactions using a GAL4 yeast two-hybrid system. Several Mate & Plate Libraries are available ready-made from Takara Bio. For libraries that are not available, this system provides the necessary components and a simple, highly efficient method to make your own Mate & Plate Library using SMART technology and the highly efficient homologous recombination machinery of Saccharomyces cerevisiae.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Library generation using n vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec.

 Library generation using n vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec.
Library generation using in vivo recombination in S. cerevisiae. Mate & Plate Libraries are created via recombination between your cDNA and the Matchmaker prey vector pGADT7-Rec. The homologues are generated by SMART cDNA synthesis. Colonies are pooled, mixed, and aliquoted into multiple vials. Each single 1 ml vial can be used for a two-hybrid screen.

Back

Mate & Plate libraries display broad insert representation

Mate & Plate libraries display broad insert representation
Mate & Plate libraries display broad insert representation. A human bone marrow library was made using the Make Your Own “Mate & Plate” Library System. Inserts from 15 randomly picked colonies were analyzed by yeast colony PCR using the Advantage 2 Polymerase Mix (Cat. No. 639201), and the Matchmaker AD LD-Insert Screening Amplimer Set (Cat. No. 630433). As seen in Lanes 1–15, every colony contained an insert of a different size. Lane M: 1 kb DNA ladder molecular weight marker.

Back

SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec

SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec
SMART cDNA synthesis generates cDNA ends with homology to pGADT7-Rec.

Back

Make Your Own Mate & Plate Library System Catalog No. 630490

Make Your Own Mate & Plate Library System Catalog No.  630490
Make Your Own "Mate & Plate™" Library System
Catalog No. 630490
.

Back

630490: Make Your Own "Mate & Plate" Library System

630490: Make Your Own "Mate & Plate" Library System

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Documents Components You May Also Like Image Data

Back

The yeast two-hybrid principle

The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.

Back

Matchmaker Gold reporter genes

Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.

Back

Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency

Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency. Interacting bait and prey fusion proteins drive the expression of four different reporters from three different GAL4-responsive promoters (M1, G1, and G2), which are stably integrated in the genome of the reporter strain, Y2H Gold. Aureobasidin A (AbA) resistance and the two auxotrophic reporters for histidine and adenine biosynthesis confer growth selection in the presence of AbA and on histidine- and adenine-deficient media, while the a-galactosidase reporter produces blue colonies in the presence of X-alpha-Gal.

Back

630489: Matchmaker Gold Yeast Two-Hybrid System

630489: Matchmaker Gold Yeast Two-Hybrid System
630482 Mate & Plate™ Library - Universal Mouse (Normalized) 2 x 1 mL USD $621.00

This yeast two-hybrid library was constructed from mouse cDNA that had been previously normalized to preferentially remove abundant cDNAs derived from high-copy-number mRNAs. The normalization process incorporates a Duplex-Specific Nuclease (DSN) treatment and SMART technology, and increases the representation of low-copy-number transcripts in the library. This reduces the number of clones that must be screened to identify positive interactions, and facilitates the identification and characterization of novel protein-protein interactions.

A universal mouse cDNA library transformed into yeast strain Y187. The library can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Use of pretransformed libraries simplifies screening

Use of pretransformed libraries simplifies screening
Use of pretransformed libraries simplifies screening.

Back

Reduction in abundance of highly expressed gene transcripts following cDNA normalization

Reduction in abundance of highly expressed gene transcripts following cDNA normalization
Reduction in abundance of highly expressed gene transcripts following cDNA normalization. cDNA normalization reduces the abundance of highly expressed gene transcripts. Data are shown for genes from mixed tissues, before and after normalization, which exhibit greater than 10,000 Mean Fluorescence Units (MFU), representing over 7,000 genes. Approximately 3,300 genes show a significant reduction in intensity, and thus abundance, following normalization. Due to the large volume of data obtained, the median MFU was plotted for groups of 100 genes before and after normalization.

Back

DSN-Normalization reduces the amount of highly abundant transcripts

DSN-Normalization reduces the amount of highly abundant transcripts
DSN-Normalization reduces the amount of highly abundant transcripts. Normalized (Lanes N) and non-normalized (Lanes C) Human Universal cDNA samples (PCR products) were electrophoresed on a 1.5% agarose gel and transferred to Hybond-N membrane. PCR-amplified probes of GAPDH and beta-actin were labeled with 32P-dATP and hybridized to the membrane.

Back

630482: Mate & Plate Library - Universal Mouse (Normalized)

630482: Mate & Plate Library - Universal Mouse (Normalized)

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Documents Components You May Also Like Image Data

Back

The yeast two-hybrid principle

The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.

Back

Matchmaker Gold reporter genes

Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.

Back

Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency

Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency. Interacting bait and prey fusion proteins drive the expression of four different reporters from three different GAL4-responsive promoters (M1, G1, and G2), which are stably integrated in the genome of the reporter strain, Y2H Gold. Aureobasidin A (AbA) resistance and the two auxotrophic reporters for histidine and adenine biosynthesis confer growth selection in the presence of AbA and on histidine- and adenine-deficient media, while the a-galactosidase reporter produces blue colonies in the presence of X-alpha-Gal.

Back

630489: Matchmaker Gold Yeast Two-Hybrid System

630489: Matchmaker Gold Yeast Two-Hybrid System
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

The yeast two-hybrid principle

The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.

Back

Matchmaker Gold reporter genes

Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.

Back

Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency

Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency
Four reporters give the Matchmaker Gold Yeast Two-Hybrid System its high stringency. Interacting bait and prey fusion proteins drive the expression of four different reporters from three different GAL4-responsive promoters (M1, G1, and G2), which are stably integrated in the genome of the reporter strain, Y2H Gold. Aureobasidin A (AbA) resistance and the two auxotrophic reporters for histidine and adenine biosynthesis confer growth selection in the presence of AbA and on histidine- and adenine-deficient media, while the a-galactosidase reporter produces blue colonies in the presence of X-alpha-Gal.

Back

630489: Matchmaker Gold Yeast Two-Hybrid System

630489: Matchmaker Gold Yeast Two-Hybrid System
630494 Yeast Media Set 2 Each USD $302.00

Complete media sets for two-hybrid screening. Highest performing yeast media-hybrid system.

Package Contents: 

  • 2 pouches YPDA Broth
  • 1 pouch YPDA with Agar
  • 1 pouch SD/-Leu Broth
  • 1 pouch SD/-Leu with Agar
  • 1 pouch SD/-Trp Broth
  • 1 pouch SD/-Trp with Agar
  • 10 pouches SD/-Leu/-Trp with Agar
  • 1 pouches SD/-Ade/-His/-Leu/-Trp with Agar
Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Yeast media pouches

Yeast media pouches
Yeast media pouches. Each ready-to-go pouch provides a precise amount of premixed media and supplements to make 0.5 L of broth or agar media. Yeast Media Sets contain a complete assortment of mixes for preparing eight specialized broth and agar media, and are designed to complement our advanced Matchmaker Gold Systems. The Yeast Media Set 2 Plus includes Aureobasidin A and X-alpha-Gal, as well as media.

Back

630494: Yeast Media Set 2

630494: Yeast Media Set 2
630495 Yeast Media Set 2 Plus Each USD $646.00

The Yeast Media Set 2 Plus contains the media and supplements needed for yeast two-hybrid library screening with the Matchmaker Gold Yeast Two-Hybrid System (Cat. No. 630489). For the ready-to-go, preformulated pouches, just add water and autoclave according to the instructions provided in the Yeast Media Protocol-at-a-Glance: no measuring, mixing, or pH adjustments are required. Directions for the use of Aureobasidin A and X-alpha-Gal are provided in the Matchmaker Gold Yeast Two-Hybrid System User Manual.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

The yeast two-hybrid principle

The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.

Back

Matchmaker Gold reporter genes

Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.

Back

Yeast media pouches

Yeast media pouches
Yeast media pouches. Each ready-to-go pouch provides a precise amount of premixed media and supplements to make 0.5 L of broth or agar media. Yeast Media Sets contain a complete assortment of mixes for preparing eight specialized broth and agar media, and are designed to complement our advanced Matchmaker Gold Systems. The Yeast Media Set 2 Plus includes Aureobasidin A and X-alpha-Gal, as well as media.
630466 Aureobasidin A 1 mg USD $221.00

Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic, isolated from the filamentous fungus Aureobasidium pullulans R106, which is toxic to yeast at low concentrations (0.1–0.5 μg/ml). Sensitive species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans and A. niger. AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, whichis expressed from the AUR1 gene. Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene

Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic that inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, expressed from the AUR1 gene. Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.

Back

630466: Aureobasidin A

630466: Aureobasidin A
630462 X-alpha-Gal 100 mg USD $179.00

X-α-Gal is a chromogenic substrate used to detect α-galactosidase activity. In yeast, α-galactosidase is a secreted reporter that is expressed from the MEL1 gene in response to GAL4 activation. α-galactosidase accumulates in the media and hydrolyses X-α-Gal causing yeast colonies to turn blue. X-α-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies

X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies
X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies.

Back

Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal

Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal
Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
630463 X-alpha-Gal 250 mg USD $309.00

X-α-Gal is a chromogenic substrate used to detect α-galactosidase activity. In yeast, α-galactosidase is a secreted reporter that is expressed from the MEL1 gene in response to GAL4 activation. α-galactosidase accumulates in the media and hydrolyses X-α-Gal causing yeast colonies to turn blue. X-α-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies

X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies
X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies.

Back

Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal

Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal
Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2022 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • PCR and qPCR hot summer sale
  • Cell-free DNA purification kit sale
  • GoStix Plus special offers
  • PCR samples
  • ThruPLEX HV special offer
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody purification and immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • DNA Day 2022
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • Applications
  • About
  • Contact us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • Diagnostic solutions
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • mRNA and cDNA synthesis
  • COVID-19 research
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Cell biology assays
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • Diagnostic solutions
  • Infectious diseases
  • Reproductive health
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Cell-free DNA purification kits
  • Viral DNA and RNA purification kits
  • Microbiome
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Stem cell services
  • Gene and cell therapy manufacturing services
  • Customer service
  • Technical support
  • Sales
  • Shipping & delivery
  • Partnering & Licensing
  • Feedback
  • Webinars from Takara Bio
  • Vector information
  • Online tools
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Webinars from Takara Bio
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • GoStix Plus FAQs
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Apollo library prep system introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Cell biology assays
  • Stem cell research
  • Overview
  • Protocols
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • FAQs
  • Citations
  • Selection guides
  • Protein research
  • Capturem technology
  • Antibody purification and immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • FAQ
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • RNA purification
  • RNA purification kit finder
  • Viral DNA and RNA purification
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • Accessory selection guides
  • Microbiome
  • Antibodies and ELISA
  • Osteocalcin focus
  • Real-time PCR
  • Overview
  • Product finder
  • Reaction size guidelines
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit
  • Cloning antibody variable regions
  • Applications
  • Molecular diagnostics
  • Pathogen detection
  • Vaccine development
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • DSS Takara Bio India Pvt. Ltd : Manufacturing
  • Our partners
  • Special offers
  • New products
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • DNA Day 2022
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Special offers
  • PCR and qPCR hot summer sale
  • Cell-free DNA purification kit sale
  • GoStix Plus special offers
  • PCR samples
  • ThruPLEX HV special offer
  • Lab Essentials
  • IVTpro mRNA Synthesis kit
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us