Aureobasidin A for yeast two-hybrid studies
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic, isolated from the filamentous fungus Aureobasidium pullulans R106, which is toxic to yeast at low concentrations (0.1–0.5 µg/ml). Sensitive species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans, and A. niger.
AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, which is expressed from the AUR1 gene (Takesako et al. 1993, Hashida-Okado et al. 1996). Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic, isolated from the filamentous fungus Aureobasidium pullulans R106, which is toxic to yeast at low concentrations (0.1–0.5 µg/ml). Sensitive species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans, and A. niger.
AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, which is expressed from the AUR1 gene (Takesako et al. 1993, Hashida-Okado et al. 1996). Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.
Perfect reporter for yeast two-hybrid studies
AbA selection virtually eliminates the high numbers of background colonies that often plague low-stringency primary screens that use nutritional markers alone (e.g. HIS3). Because AbA actually kills sensitive yeast cells, rather than merely retarding their growth, AbA-based selection greatly favors the growth and identification of genuinely positive clones. In general practice, a high percentage of clones that emerge from low-stringency primary screens using AbA selection alone, are subsequently verified on high-stringency secondary screens that select for all four Matchmaker Gold reporters (AUR1-C, HIS3, ADE2 and MEL1).
Overview
- Stringent drug-selectable marker for yeast
- Aureobasidin A resistance is an ideal reporter for yeast two-hybrid studies
- Provides exceptional screening results with Matchmaker Gold Yeast One- and Two-Hybrid Systems
More Information
Applications
- Yeast two-hybrid studies
- Yeast one-hybrid studies
References
Hashida-Okado, T. et al. AUR1, a novel gene conferring aureobasidin resistance on Saccharomyces cerevisiae: a study of defective morphologies in Aur1p-depleted cells. Mol. Gen. Genet. 251(2):236-244 (1996).
Takesako, K. et al. Biological properties of aureobasidin A, a cyclic depsipeptide antifungal antibiotic. J. Antibiot. (Tokyo) 46(9):1414–20 (1993).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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