Yeast media, amino acid dropout mixes
These high-quality dropout supplements can be used together with SD base to create selective media for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker Gold Systems and other yeast one-hybrid systems or yeast two-hybrid systems.
These high-quality dropout supplements can be used together with SD base to create selective media for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker Gold Systems and other yeast one-hybrid systems or yeast two-hybrid systems.
Dropout supplements
Dropout (DO) supplements provide various mixtures of amino acids and nucleotides to add to the Minimal SD Bases to make a defined minimal medium. In each DO mix, certain amino acids have been “dropped out”, for example the -Leu/-Trp DO supplement contains every essential amino acid except for leucine and tryptophan. DO Supplements may also be added to the minimal E. coli medium M9 for minimal selection in E. coli. Each package will make at least 10 L of medium.
Overview
- Bulk media quantities for culturing Saccharomyces cerevisiae
- Dropout (DO) supplements for use with SD base to create selective media
- Proven track record—15 years of yeast media sales
More Information
Applications
- Confirmation of auxotrophic phenotypes
- Selection for yeast transformants
- Yeast two-hybrid screening
- Yeast one-hybrid screening
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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