X-alpha-Gal for yeast two-hybrid studies
X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar.
The X-alpha-Gal assay detects the activation of the yeast MEL1 gene, a GAL4-regulated gene used in yeast two-hybrid screening. MEL1 encodes the secreted enzyme alpha-galactosidase, which hydrolyzes colorless X-alpha-Gal into a blue end product. Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
- Colonies turn blue with positive yeast two-hybrid interactions
- Direct visualization on the plate, no lysis required
- Yeast two-hybrid studies (Matchmaker Gold)
Aho, S. et al. A novel reporter gene MEL1 for the yeast two-hybrid system. Anal. Biochem. 253, 270–272 (1997).
Liljestrom, P. L. The nucleotide sequence of the yeast MEL1 gene. Nucleic Acids Res. 13, 7257–7268 (1985).
Additional product information
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