Two-hybrid and one-hybrid systems
X-alpha-Gal for yeast two-hybrid studies
X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar.
The X-alpha-Gal assay detects the activation of the yeast MEL1 gene, a GAL4-regulated gene used in yeast two-hybrid screening. MEL1 encodes the secreted enzyme alpha-galactosidase, which hydrolyzes colorless X-alpha-Gal into a blue end product. Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
- Colonies turn blue with positive yeast two-hybrid interactions
- Direct visualization on the plate, no lysis required
- Yeast two-hybrid studies (Matchmaker Gold)
Aho, S. et al. A novel reporter gene MEL1 for the yeast two-hybrid system. Anal. Biochem. 253, 270–272 (1997).
Liljestrom, P. L. The nucleotide sequence of the yeast MEL1 gene. Nucleic Acids Res. 13, 7257–7268 (1985).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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