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Yeast minimal media—SD base

Synthetic defined (SD) base, minimal base including glucose and yeast nitrogen base minus amino acids

These high-quality yeast growth media can be used together with dropout (DO) supplements for confirming auxotrophic phenotypes and selecting yeast transformants. They are ideal for use with Matchmaker Gold systems and other yeast one-hybrid systems or yeast two-hybrid systems.

Minimal SD bases

Minimal synthetic defined (SD) bases include a yeast nitrogen base, ammonium sulfate, and a glucose as a carbon source. Amino acids are not added so dropout (DO) supplements must be added separately to the Minimal SD Base to make a synthetic, defined medium lacking the specified nutrients allowing for selection of transformed yeast clones. The SD bases are available with or without agar premixed in the medium. Each package of Minimal SD Base makes 10 liters of medium.

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Overview

Yeast minimal media for culturing Saccharomyces cerevisiae
SD base for use with dropout (DO) supplements
Proven track record—15 years of yeast media sales

More Information

Applications

Selection of auxotrophic phenotypes in yeast
Selection for plasmid after yeast transformation
Yeast two-hybrid screening
Yeast one-hybrid screening

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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