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Yeast minimal media—SD base

Synthetic defined (SD) base, minimal base including glucose and yeast nitrogen base minus amino acids

These high-quality yeast growth media can be used together with dropout (DO) supplements for confirming auxotrophic phenotypes and selecting yeast transformants. They are ideal for use with Matchmaker Gold systems and other yeast one-hybrid systems or yeast two-hybrid systems.

Minimal SD bases

Minimal synthetic defined (SD) bases include a yeast nitrogen base, ammonium sulfate, and a glucose as a carbon source. Amino acids are not added so dropout (DO) supplements must be added separately to the Minimal SD Base to make a synthetic, defined medium lacking the specified nutrients allowing for selection of transformed yeast clones. The SD bases are available with or without agar premixed in the medium. Each package of Minimal SD Base makes 10 liters of medium.

Cat. # Product Size License Quantity Details
630412 Minimal SD Agar Base 467 g USD $263.00

These high-quality yeast growth media and dropout supplements can be used for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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630412: Minimal SD Agar Base

630412:  Minimal SD Agar Base
630411 Minimal SD Base 267 g USD $185.00

These high-quality yeast growth media and dropout supplements can be used for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Clontech Bulk Media

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Clontech Bulk Media.

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630411: Minimal SD Base

630411: Minimal SD Base

Yeast two-hybrid products Yeast two-hybrid products
Quick and easy yeast transformation kit Quick & easy yeast transformation
Amino acids mixes for yeast minimal media Amino acid dropout mixes

Overview

  • Yeast minimal media for culturing Saccharomyces cerevisiae
  • SD base for use with dropout (DO) supplements
  • Proven track record—15 years of yeast media sales

More Information

Applications

  • Selection of auxotrophic phenotypes in yeast
  • Selection for plasmid after yeast transformation
  • Yeast two-hybrid screening
  • Yeast one-hybrid screening

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Related yeast products

  • Matchmaker Gold Yeast Two-Hybrid System
  • Normalized Mate & Plate libraries
  • Mate & Plate libraries
  • Yeast one-hybrid system
  • Two-hybrid library construction kit
  • Yeast two-hybrid media
  • Yeast one-hybrid media
  • Minimal media pouches (ready-mixed)
  • YPDA and YPD rich media
  • Amino acid dropout mixes
  • Quick & Easy Yeast Transformation Mix
  • Yeastmaker transformation system
  • Yeast plasmid-isolation minipreps
  • Aureobasidin A
  • X-alpha-Gal

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  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
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  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
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  • Identification and characterization
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  • Food crop pathogens
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  • Sample prep from plasma
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