Yeast two-hybrid cDNA libraries—Mate & Plate
We strongly recommend the use of our Mate & Plate and normalized Mate and Plate Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast two-hybrid screening because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.
We strongly recommend the use of our Mate & Plate and normalized Mate and Plate Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast two-hybrid screening because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.
To screen, simply take a 1-ml library vial and culture overnight with a Y2H Gold clone that expresses your bait protein, and then plate on selective media. A single Mate & Plate Library aliquot is sufficient for the complete screening of more than one million independent clones.
Or do it yourself!
Alternatively you can use our Make Your Own “Mate & Plate” Library System (Cat. # 630490) to make your own customized library. With this system, you can generate enough 1 ml-library aliquots for more than 100 screens.
Or you can upgrade further with these specialized Libraries:
- Normalized Mate & Plate Libraries
- Mixed-tissue Mate & Plate Universal Libraries
Overview
- The simplest way to screen a yeast two-hybrid library
- Highly complex, tissue-specific libraries
More Information
Applications
- Yeast two-hybrid screening
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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