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Yeast two-hybrid system

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Yeast two-hybrid cDNA libraries—Mate & Plate

We strongly recommend the use of our Mate & Plate and normalized Mate and Plate Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast two-hybrid screening because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.

We strongly recommend the use of our Mate & Plate and normalized Mate and Plate Libraries with the Matchmaker Gold Yeast Two-Hybrid System. These libraries provide, by far, the simplest method for yeast two-hybrid screening because they do not require library-scale transformations or labor-intensive library amplification. Thus, very little optimization or hands-on time is required with these libraries.

To screen, simply take a 1-ml library vial and culture overnight with a Y2H Gold clone that expresses your bait protein, and then plate on selective media. A single Mate & Plate Library aliquot is sufficient for the complete screening of more than one million independent clones.

Or do it yourself!

Alternatively you can use our Make Your Own “Mate & Plate” Library System (Cat. # 630490) to make your own customized library. With this system, you can generate enough 1 ml-library aliquots for more than 100 screens.

Or you can upgrade further with these specialized Libraries:

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Cat. # Product Size License Quantity Details
630478 Mate & Plate™ Library - Mouse Embryo 11-day 5 x 1 mL USD $1239.00

Mouse embryo (11-day) cDNA library transformed into yeast strain Y187. The library can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Documents Components You May Also Like Image Data
630476 Mate & Plate™ Library - Mouse Embryo 17-day 5 x 1 mL USD $1239.00

Mouse embryo (17-day) cDNA library transformed into yeast strain Y187. The library can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Documents Components You May Also Like Image Data
630471 Mate & Plate™ Library - Human Heart 5 x 1 mL USD $1239.00

Human heart cDNA library transformed into yeast strain Y187. The library can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Documents Components You May Also Like Image Data
630470 Mate & Plate™ Library - Human Testis 5 x 1 mL USD $1239.00

Human testis cDNA library transformed into yeast strain Y187. The library can be readily mated to a MATa GAL4 reporter strain, such as AH109 or Y2HGold.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Required Products

Cat. # Product Size Price License Quantity Details
630489 Matchmaker® Gold Yeast Two-Hybrid System Each USD $989.00

Complete GAL4-based two-hybrid system to detect protein interactions. Yeast strain Y2HGold facilitates the elimination of many false positives that arise when screening an activation domain (AD) fusion library, because it contains four separate reporter genes that can be used to select for protein interactions. The system is also characterized by low background due to the Aureobasidin A resistance marker.

Documents Components You May Also Like Image Data

Overview

  • The simplest way to screen a yeast two-hybrid library
  • Highly complex, tissue-specific libraries

More Information

Applications

  • Yeast two-hybrid screening

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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