Yeast plasmid-isolation kit

The Easy Yeast Plasmid Isolation Kit provides a simple and efficient miniprep method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.

Cat. #	Product	Size		License	Quantity	Details
630467	Easy Yeast Plasmid Isolation Kit	50 Preps	USD $373.00
This kit provides an easy and efficient method to rescue plasmid DNA from Saccharomyces cerevisiae. The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts, and spin column purification of the plasmid DNA. Purified DNA can then be transformed into E. coli for propagation and purification. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR. Back 630467: Easy Yeast Plasmid Isolation Kit

Overview

Rescue plasmids from yeast using spin columns
Highly efficient Zymolyase is included
Purify DNA in less time

More Information

Applications

Plasmid DNA minipreps from yeast
Rescue positive prey plasmids after a yeast two-hybrid or yeast one-hybrid screen

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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Yeast plasmid-isolation kit

Notice to purchaser

The Easy Yeast Plasmid Isolation protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts

630467: Easy Yeast Plasmid Isolation Kit

Overview

More Information

Applications

Additional product information