- Purification products
- Two-hybrid and one-hybrid systems
- Mass spectrometry reagents
- Expression vectors & systems
- Antibodies and immunoprecipitation
- SDS-PAGE & western blotting
- Protein quantification
- Protein sequencing
- Accessory enzymes
Yeast plasmid-isolation kit
The Easy Yeast Plasmid Isolation Kit provides a simple and efficient miniprep method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses Zymolyase for highly efficient enzymatic disruption of yeast cell walls, followed by SDS/alkaline lysis of the resulting spheroplasts. A spin column is used to purify the plasmid DNA which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.
- Rescue plasmids from yeast using spin columns
- Highly efficient Zymolyase is included
- Purify DNA in less time
- Plasmid DNA minipreps from yeast
- Rescue positive prey plasmids after a yeast two-hybrid or yeast one-hybrid screen
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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