Sphingolipid hydrolysis: Sphingolipid ceramide N-deacylase (SCDase)
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.
More Information
Applications
- Hydrolysis of N-acyl linkages between fatty acids and sphingosine bases
- Sphingolipid hydrolysis
Source
Pseudomonas sp.
Storage
Store at −20°C until use. Store reconstituted solution in aliquots at −20°C. Avoid freeze-thaw.
Properties
- Source: Pseudomonas sp.
- Concentration: 5 mU/µl
- Inhibitors: Hg2+, Zn2+, and Cu2+
- Not inhibited by: MN2+, Ca2+, Mg2+, or EDTA
- Optimum pH:
- Forward reaction: pH 5.0–6.0 (0.8% TRITON X-100)
- Reverse reaction: pH 7.0 (0.1% TRITON X-100)
Form
Solution in 50 mM sodium acetate (pH 6.0) containing 0.1% Lubrol PX
Product citations
Ito, M. et al., A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms. J. Biol. Chem. 270, 24370-24374 (1995).
Mitsutake, S. et al., [14C]ceramide synthesis by sphingolipid ceramide N-deacylase: new assay for ceramidase activity detection. Anal. Biochem. 247,52-57 (1997).
Mitsutake, S. et al., Enzymatic synthesis of 14C-glycosphingolipids by reverse hydrolysis reaction of sphingolipid ceramide N-deacylase: detection of endoglycoceramidase activity in a seaflower. J. Biochem. (Japan) 123:859–863 (1998).
Tani, M. et al., Enzymatic synthesis of omega-amino-ceramide: preparation of a sensitive fluorescent substrate for ceramidase. Anal. Biochem. 263,183–188 (1998).
Definition of activity
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 µmol of asialo GM1 per minute.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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