- Purification products
- Two-hybrid and one-hybrid systems
- Mass spectrometry reagents
- Expression vectors & systems
- Antibodies and immunoprecipitation
- SDS-PAGE & western blotting
- Protein quantification
- Protein sequencing
- Accessory enzymes
Sphingolipid hydrolysis: Sphingolipid ceramide N-deacylase (SCDase)
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.
- Hydrolysis of N-acyl linkages between fatty acids and sphingosine bases
- Sphingolipid hydrolysis
Store at −20°C until use. Store reconstituted solution in aliquots at −20°C. Avoid freeze-thaw.
- Source: Pseudomonas sp.
- Concentration: 5 mU/µl
- Inhibitors: Hg2+, Zn2+, and Cu2+
- Not inhibited by: MN2+, Ca2+, Mg2+, or EDTA
- Optimum pH:
- Forward reaction: pH 5.0–6.0 (0.8% TRITON X-100)
- Reverse reaction: pH 7.0 (0.1% TRITON X-100)
Solution in 50 mM sodium acetate (pH 6.0) containing 0.1% Lubrol PX
Ito, M. et al., A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms. J. Biol. Chem. 270, 24370-24374 (1995).
Mitsutake, S. et al., [14C]ceramide synthesis by sphingolipid ceramide N-deacylase: new assay for ceramidase activity detection. Anal. Biochem. 247,52-57 (1997).
Mitsutake, S. et al., Enzymatic synthesis of 14C-glycosphingolipids by reverse hydrolysis reaction of sphingolipid ceramide N-deacylase: detection of endoglycoceramidase activity in a seaflower. J. Biochem. (Japan) 123:859–863 (1998).
Tani, M. et al., Enzymatic synthesis of omega-amino-ceramide: preparation of a sensitive fluorescent substrate for ceramidase. Anal. Biochem. 263,183–188 (1998).
Definition of activity
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 µmol of asialo GM1 per minute.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.