His-tagged recombinant protein purification—His60 Ni Gravity Columns

His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. His60 Ni Resin is compatible with batch/gravity flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

His60 Ni Gravity Columns are prepacked columns containing 1 ml of resin. The His60 Ni Gravity Column Purification Kit supplies all buffers needed for protein extraction and purification, plus prepacked His60 Ni Gravity Columns. These buffers are also available separately in the His60 Ni Buffer Set.

The recombinant his-tagged protein purification principle

His60 Ni Superflow purification resin has low metal ion-leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins). The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal-ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins.

The recombinant his-tagged protein purification procedure

Purifying recombinant his-tagged proteins requires cell lysis/extraction, binding, washing, and elution. His60 Ni makes protein purification under either native or denaturing conditions a one-step process. The resin is compatible with multiple denaturants and detergents. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH.