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Capturem high-capacity membranes

Capturem high-capacity membranes

Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells.

Capturem nickel-functionalized membranes for his-tagged purification are a revolutionary solution for recombinant protein purification that effortlessly outperforms conventional methods. Novel nylon membranes have been assembled into spin columns and filtration devices, creating no-waiting, room-temperature workflows (just 5–30 minutes from loading a sample through elution) compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., BME, EDTA, DTT, glycerol, TCEP, etc.; see compatibility table), and when performing tandem purification of his-tagged proteins.

The modified nylon membranes have a small bed volume, yet still produce high-purity, concentrated protein, due to the chemically enhanced surface area of the pores. With this technology, protein-binding capacity is comparable to, or better than, resins that bind 75 mg or more per cm3 of membrane. Miniprep, maxiprep, 24-well-plate, 96-well-plate, and large-volume formats are available to accommodate a range of input volumes. Furthermore, the short residence time of protein samples greatly reduces the possibility of protein degradation or loss of activity, giving downstream applications a higher probability of success.

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Cat. # Product Size License Quantity Details
635710 Capturem™ His-Tagged Purification Miniprep Kit 20 purifications USD $215.00

The Capturem His-Tagged Purification Miniprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 850 μl of clarified lysate. The columns are suitable for use under native or denaturing conditions, in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Each column requires a minimum elution volume of 100 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635713 Capturem™ His-Tagged Purification Maxiprep Kit 6 Purifications USD $280.00

The Capturem His-Tagged Purification Maxiprep Kit provides single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 23 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Each column requires a minimum elution volume of 500 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635719 Capturem™ His-Tagged Purification Maxiprep Columns 50 Columns USD $1028.00

Capturem His-Tagged Purification Maxiprep Columns are single-use disposable membrane spin columns for simple, rapid purification of his-tagged proteins in up to 23 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions, and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Each column requires a minimum elution volume of 500 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635724 Capturem™ His-Tagged Purification Large Volume 4 Units (2 Units per box) USD $460.00

The Capturem His-Tagged Purification Large Volume unit lets you quickly purify up to 25 mg of protein (depending on protein size and expression level) from a large volume of lysate (up to 500 ml at a time), resulting in purified protein concentrated at up to 2 mg/ml. Like our other his-tagged Capturem products, the Large Volume unit works for bacterial and mammalian expression systems with a wide range of additives. Each unit requires a minimum elution volume of 1.5 ml.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635730 Capturem™ His-Tagged Purification 24-Well Plate 1 x 24-well plate USD $410.00

Capturem His-tagged 24-Well Plates are 24-well plates that provide rapid, high-quality, small-scale purification of his-tagged proteins. These plates combine Ni-NTA chemistry with novel Capturem technology to provide high-capacity, membrane-based affinity purification. Typical yields for his-tagged proteins are up to 800 µg per well with a 4.5-ml sample capacity per well.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635714 Capturem™ His-Tagged Purification 96-Well Plate 1 x 96-well plate USD $403.00

Capturem His-Tagged Purification 96 is a single-use, disposable 96-well plate for simple, rapid purification of his-tagged proteins from up to 1 ml of clarified lysate per well. This plate is suitable for use under native or denaturing conditions, in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Capturem His-Tagged Purification 96 is compatible with automated liquid handlers equipped with vacuum filtration or centrifuges equipped with appropriate plate holders. Each well requires a minimum elution volume of 100 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635715 Capturem™ His-Tagged Purification Maxiprep Columns 25 Columns USD $647.00

Capturem His-Tagged Purification Maxiprep Columns are single-use, disposable, membrane spin columns for simple, rapid purification of his-tagged proteins in up to 23 ml of clarified lysate. The columns are suitable for use under native or denaturing conditions and in the presence of additives such as DTT (up to 10 mM), βME (up to 30 mM), TCEP (up to 5 mM), EDTA (up to 10 mM), or glycerol. Each column requires a minimum elution volume of 500 µl.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Overview

  • No-waiting workflow—5-minute, room-temperature protocol
  • High purity and yield—small column bed volumes trap fewer contaminants and allow large-volume washes
  • Compatible with wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, BME, glycerol, TCEP, etc.; see compatibility table)
  • Lysis buffer compatibility—xTractor Buffer; buffers from other vendors
  • Different cell systems—easy purification from mammalian and bacterial cells

More Information

Applications

  • Biochemical/enzymatic assays
  • Pull-down or other protein interaction assays
  • Confirmation screening
  • Protein/ligand affinity analysis
  • Crystallography or other structural analyses
  • Antibody production
  • Toxicology studies
  • Protein transfer to live cells

Approximate yields for Capturem his-tagged formats

Format Yield* Purification time
Miniprep 80 µg 5 min
96-well plate 80 µg 15 min
24-well plate 800 µg 15 min
Maxiprep 1.5 mg 15 min
Large volume 8–20 mg 30 min

*Yields vary depending upon the sample type, species, and antibody isotype.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Tech note

A revolutionary solution for recombinant protein purification

Learn about a novel solution for recombinant protein purification from mammalian and bacterial cell lysates.

Capturem technology overview

Capturem his-tagged purification

Simplified purification of active, secreted his-tagged proteins

Purify active, secreted his-tagged proteins from cell-culture supernatants in 15 minutes.

Tech note: Purification of active, secreted proteins

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