Capturem nickel column reagent compatibility for his-tagged purification
Overview
Capturem his-tagged purification columns utilize high-capacity Ni-NTA membranes to quickly purify concentrated recombinant proteins under a wide range of conditions. These columns are compatible with a wide range of lysis buffers including our xTractor Buffer, which is provided with the miniprep and maxiprep purification kits. Purification results are consistent regardless of native or denaturing conditions or the presence of additives (e.g., EDTA, ßME, TCEP, and DTT).
| Reagents compatible with Capturem nickel columns | |
|---|---|
| Reagent | Compatibility* |
| MOPS | 200 mM |
| HEPES | 200 mM |
| Tris | 200 mM |
| EDTA | 10 mM |
| Beta-mercaptoethanol | 30 mM |
| DTT | 10 mM |
| TCEP | 5 mM |
| Guanidine-HCl | 6 M |
| Urea | 8 M |
| Nonionic detergent (Triton X-100) | 2% |
| Nonionic detergent (Tween 20) | 2% |
| Anionic detergent (SDS) | 1% |
| Arginine | 500 mM |
| Glycine | 100 mM |
| Histidine | 20 mM |
| Sodium chloride | 2 M |
| Imidazole | 40 mM |
| Glycerol | 10% |
* Compatible with concentrations up to the amounts shown. These are the maximum amounts that have been tested.
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