- Why tag a protein?
- Tech note: cobalt resin
- Purification methods overview
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- TALON resin selection guide
- Selection guide: His60 resin
- FAQs: TALON
- Video: Capturem his maxiprep
- Video: Capturem his miniprep
- Visual protocol: Capturem his maxiprep
- Visual protocol: Capturem his miniprep
- Capturem nickel column reagent compatibility
- TALON reagent compatibility
- His60 reagent compatibility
- TALON: Native vs denaturing purification
- Protocol: denaturing purification with TALON resin, pH elution
- Protocol: native purification with TALON resin, imidazole elution
- Protocol: native purification with TALON resin, pH elution
Next-generation his-tagged protein purification in a maxiprep format
Get high yields of highly concentrated his-tagged proteins, in only 15 minutes, with the Capturem His-Tagged Purification Maxiprep Kit.
Watch this short video to see how it works!
- No-waiting workflow—15-minute, room-temperature protocol, from sample application through elution
- High purity and yield—approximately 2.5 mg/column
- Elute concentrated protein—up to 4.5 mg/ml
- Wide range of conditions—native and denaturing conditions; with common additives (e.g., EDTA, DTT, βME, glycerol, TCEP, etc.)
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