High-capacity his-tagged protein purification with His60 Ni resin

Cobalt resin products

His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. His60 Ni resin is available in a variety of formats. Use the following table to decide which one is best for your specific application.

Choose the best His60 resin format for your application
	His60 Ni Superflow Resin	His60 Ni Superflow Cartridges	His60 Ni Gravity Columns	His60 Ni Magnetic Beads
Format	Bulk package	FPLC cartridge	1-ml column	Magnetic beads
Binding capacity	60 mg protein/ml resin	60 mg protein/ml resin	60 mg protein/ml resin	33 mg protein/ml resin
Starting material	Clarified lysate	Clarified lysate	Clarified lysate	Clarified lysate
FPLC applications	Yes	Yes	No	No
Compatible with LC systems, including ÄKTA	Yes	Yes	No	No
Time savings	+	+++	+++	+++
Maximum pressure	10 bar 0.97 MPa	5 bar 0.5 MPa	N/A	N/A
Maximum flow rate (ml/min)	50	10 (1-ml cartridge) 40 (5-ml cartridge)	N/A	N/A
Bead size (µm)	60–160	60–160	60–160	20–75
Downstream applications	Preliminary protein characterization Fast capture of his-tagged proteins	Protein labeling Immunization to produce antibodies Animal studies	Protein labeling Immunization to produce antibodies Animal studies	Microscale protein purification Scaleable purification protocols Simultaneously purify several proteins Verify his-tagged protein expression in transformants

His60: high-capacity Ni-IDA resins

His60 nickel resin beats Ni-NTA resin for his-tag purification

Discover how to improve protein purity from Ni resin purification

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