- Capturem technology
- Antibody purification
- Why tag a protein?
- Tech note: cobalt resin
- Purification methods overview
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- TALON resin selection guide
- Selection guide: His60 resin
- FAQs: TALON
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Mass spectrometry digestion reagents
- Matchmaker Gold yeast two-hybrid systems
- Expression systems
High-capacity his-tagged protein purification with His60 Ni resin
His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. His60 Ni resin is available in a variety of formats. Use the following table to decide which one is best for your specific application.
|Choose the best His60 resin format for your application|
|His60 Ni Superflow Resin||His60 Ni Superflow Cartridges||His60 Ni Gravity Columns||His60 Ni Magnetic Beads|
|Format||Bulk package||FPLC cartridge||1-ml column||Magnetic beads|
||60 mg protein/ml resin||60 mg protein/ml resin||60 mg protein/ml resin||33 mg protein/ml resin|
|Starting material||Clarified lysate||Clarified lysate||Clarified lysate||Clarified lysate|
|Compatible with LC systems, including ÄKTA||Yes||Yes||No||No|
|Maximum flow rate (ml/min)||50||
|Bead size (µm)||60–160||60–160||60–160||20–75|
His60: high-capacity Ni-IDA resins
Discover how to improve protein purity from Ni resin purification
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