High-capacity his-tagged protein purification with His60 Ni resin
His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the one-step purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. The modified Ni-IDA chemistry makes this resin one of the best on the market in terms of capacity, metal ion leakage, and purity, when compared to other Ni-based resins such as Ni-NTA or other Ni-IDA resins. His60 Ni resin is available in a variety of formats. Use the following table to decide which one is best for your specific application.
Choose the best His60 resin format for your application | ||||
---|---|---|---|---|
His60 Ni Superflow Resin | His60 Ni Superflow Cartridges | His60 Ni Gravity Columns | His60 Ni Magnetic Beads | |
Format | Bulk package | FPLC cartridge | 1-ml column | Magnetic beads |
Binding capacity |
60 mg protein/ml resin | 60 mg protein/ml resin | 60 mg protein/ml resin | 33 mg protein/ml resin |
Starting material | Clarified lysate | Clarified lysate | Clarified lysate | Clarified lysate |
FPLC applications | Yes | Yes | No | No |
Compatible with LC systems, including ÄKTA | Yes | Yes | No | No |
Time savings | + | +++ | +++ | +++ |
Maximum pressure |
|
|
N/A | N/A |
Maximum flow rate (ml/min) | 50 |
|
N/A | N/A |
Bead size (µm) | 60–160 | 60–160 | 60–160 | 20–75 |
Downstream applications |
|
|
|
|
His60: high-capacity Ni-IDA resins
His60 nickel resin beats Ni-NTA resin for his-tag purification
Discover how to improve protein purity from Ni resin purification
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2024 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.