We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to Antibody immunoprecipitation
  • IP and Co-IP of cardiac voltage-gated ion channel proteins
  • Tech note: thiophilic antibody purification resins
  • Tech note: IP and Co-IP
Thiophilic resin Thiophilic resin
Home › Learning centers › Protein research › Antibody immunoprecipitation › Tech note: thiophilic antibody purification resins

Protein research

  • Capturem technology
    • Capturem protocols
    • Capturem tech notes and applications
    • FAQs about Capturem technology
    • Capturem technology citations
    • Capturem posters
  • Antibody immunoprecipitation
    • IP and Co-IP of cardiac voltage-gated ion channel proteins
    • Tech note: thiophilic antibody purification resins
    • Tech note: IP and Co-IP
  • His-tag purification
    • Purification methods overview
    • TALON resin selection guide
    • Selection guide: His60 resin
    • xTractor Buffer is optimized for superior protein yield
    • Why tag a protein?
    • Tech note: cobalt resin
    • Simplified purification of active, secreted his-tagged proteins
    • Overview: His60
    • Tech note: Capturem technology
    • Tech note: Capturem large volume
    • Magnetic beads
    • FAQs: TALON
    • Protocols
      • Video: Capturem his maxiprep
      • Video: Capturem his miniprep
      • Visual protocol: Capturem his maxiprep
      • Visual protocol: Capturem his miniprep
      • Capturem nickel column reagent compatibility
      • TALON reagent compatibility
      • His60 reagent compatibility
      • TALON: Native vs denaturing purification
      • Protocol: denaturing purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, imidazole elution
      • Protocol: native purification with TALON resin, pH elution
  • Other tag purification
    • Streptavidin-based enrichment using Capturem technology
    • Selection guide: peptide tags
    • Myc-tagged protein purification overview
    • GST-tagged protein purification overview
  • Phosphoprotein and glycoprotein purification
    • Non-tagged protein purification overview
    • Phosphoprotein purification overview
  • Matchmaker Gold yeast two-hybrid systems
    • Matchmaker Gold Yeast Two-Hybrid System
    • Make your own library for yeast two-hybrid screening
    • Mate and Plate yeast two-hybrid cDNA libraries
    • Aureobasidin A for improved selectable drug resistance in yeast
  • Expression systems
    • Protein expression overview
    • Insect expression overview
    • Mammalian expression overview
    • pHEK293 Ultra expression overview
    • OKT3 expression in mammalian cells
    • Bacterial expression overview
New products
Need help?
Contact Sales
Thiophilic resin Thiophilic resin
Tech Note

Thiophilic antibody purification resins

An efficient, versatile, and economical alternative to Protein A purification

Our Thiophilic-Superflow Resin provides highly stable purified antibodies with the following advantages over conventional Protein A antibody purification methods:

  • Purification at neutral pH—avoid antibody aggregates
  • High capacity (20–25 mg Ab/ml resin)—get high recoveries
  • Broader selectivity—purify IgY, IgM, IgE, and single-chain antibodies (scAb)
  • Fewer purification steps—save time
  • Reusable resin—save money
  • Higher stability of the purified product
Introduction Results References

Introduction  

Protein A vs. thiophilic resin

Historically, Protein A has been the preferred method of immunoglobulin purification. However, there are certain types of antibodies, such as the single-chain antibodies IgE, IgY, and IgM, that cannot be purified using Protein A. Thiophilic adsorption chromatography is ideal for these types of applications, as well as immunoglobulin purification in general. In addition to providing broader selectivity and higher stability, thiophilic adsorption chromatography offers a faster protocol than Protein A under neutral buffer conditions (Figure 1). Purification with thiophilic Resin allows elution in any buffer with a pH of 7.0. In contrast, Protein A requires elution in a buffer with a pH lower than 7.0. Protein A also requires a dialysis step, which can be omitted with thiophilic resin.

Thiophilic Resin purifies antibodies at neutral pH and in less time than Protein A

Figure 1. Thiophilic resin purifies antibodies at neutral pH and in less time than Protein A.

Results  

Thiophilic ligand allows salt-dependent purification of a wide range of antibody types

Thiophilic adsorption chromatography (TAC) was developed by Porath, et al., in 1984. TAC is a group-specific, salt-dependent purification technique with distinct adsorption affinity towards immunoglobulins and alpha-2-macroglobulins. The term "thiophilic" refers to the affinity that proteins have for sulfone groups that lie in close proximity to thioether groups (Figure 2; Porath 1985). With this technique, protein adsorbs to a sulfone thioether ligand.

The adsorption of different proteins can be promoted by adding different salts to the mixture. Varying the concentration of the loading salt can affect the adsorption affinities of IgG, IgM, IgA, Fab and Fc fragments, and C3 and C4 complement factors. Several sulfate salts can be used to promote the adsorption of target proteins. The most commonly used salts are potassium sulfate, sodium sulfate, and ammonium sulfate. In addition, salt concentration can differentially affect the adsorption kinetics of IgG, IgM, IgA, Fab and Fc fragments, and complement factors C3 and C4 (Lutomski et al. 1995; Oscarsson et al. 1992; Schulze et al. 1994; Yurov et al. 1994).

TAC is an economical technique for purifying immunoglobulins from whole serum and tissue cultures (Porath & Belew 1987; Scoble & Scopes 1997). In comparison to Protein A-based immunoadsorbents, thiophilic adsorbents have broader affinity towards immunoglobulins (Hutchens & Porath 1986). Furthermore, >99% of total proteins are recovered using a thiophilic adsorbent in comparison to less than 92% for phenyl and 75% for octyl agarose adsorbents (Oscarsson et al. 1995).

Purification of single-chain antibodies

Recombinant, single-chain antibodies are becoming increasingly common in research use because they can be genetically manipulated to bind different proteins and to perform specific, desired functions. However, standard antibody purification methods such as Protein A and Protein G do not work well for single-chain antibodies because these antibodies lack the Fc domain that natural antibodies possess. Protein A usually binds to this Fc domain. Because thiophilic resin binds to a region other than the Fc domain on single chain antibodies, it is able to purify them (Figure 3).

Figure 2. Structure of thiophilic resin.

Figure 2. Structure of thiophilic resin.

 

Figure 3. Single-chain antibody purification with thiophilic resin.

Figure 3. Single-chain antibody purification with thiophilic resin. SDS-PAGE analysis of the following samples: bacterial lysate expressing scFv215 (Lane 2), periplasmic fraction (Lane 3), peak fraction from Ni-NTA (Lane 4) and peak fraction from thiophilic resin (Lane 5). (Shultze, et al. 1994; permission to reprint obtained).

Purification of IgY

Generating antibodies in chickens rather than rabbits is becoming a common method of immunoglobulin production. Antibodies produced in immunized chickens, which are transferred to the egg yolk, can be collected daily from eggs rather than animal serum. Also, chicken egg yolk provides higher amounts of a specific immunoglobulin than rabbit serum (Hansen et al. 1998). Standard immunoglobulin purification methods do not work well for IgY because it does not adsorb to Protein A. In contrast, IgY does adsorb to thiophilic resin (Figure 4), which is ideal for this type of purification because it provides a fast, simple, and inexpensive way to obtain large amounts of purified IgY.

Figure 4. Purification of IgY from chicken egg using thiophilic resin. Panel A.

Figure 4. Purification of IgY from chicken egg using thiophilic resin. Panel A. SDS-PAGE analysis of fractions from purification of chicken egg immunoglobulins. Lanes 7 & 10 each contain 10 mg of protein, and all other lanes, 25 mg each. Lane 1: Egg yolk extract supernatant. Lane 2: Supernatant after 60% SAS (Saturated concentration of Ammonium Sulfate). Lane 3: Wash with 60% SAS. Lane 4: Pellet after 60% SAS. Lane 5: Column load. Lane 6: Unbound material. Lane 7: Eluted material. Lanes 8–10: Purification with another thiophilic resin. Panel B. Immunoblot using 1/10 the amount of material in Panel A. IgY was detected with polyclonal rabbit anti-chicken HRP-conjugate. M = molecular weight. (Hansen et al. 1998; permission to reprint obtained).

Flexibility: suitable for batch, gravity-flow, or FPLC applications

Thiophilic-Superflow Resin (Table I) can be used for batch and gravity-flow purification, as well as FPLC. This resin is prepared using Superflow 6 agarose crosslinked beads, which permit linear flow rates as high as 5 cm/min. The agarose beads were activated with divinylsulfone, and mercaptoethanol was coupled to the activated resin. This resin can be regenerated and reused without detrimental effects on specificity and capacity.

Figure 5. Thiophilic-Superflow Resin purifies IgG at a high flow rate and neutral pH.

Figure 5. Thiophilic-Superflow Resin purifies IgG at a high flow rate and neutral pH. Whole rabbit serum in 0.5 M sodium sulfate was purified on a Thiophilic-Superflow Resin column and eluted with 0.05 M sodium sulfate (Peak II).

Table I: Properties of Thiophilic-Superflow Resin
Batch/gravity Yes
FPLC Yes
Scale Analytical, preparative
Preparative production capacity (mg Ig/ml absorbent) 25
Matrix Cross-linked agarose
Maximum linear flow rate (cm/min) 5.0
Maximum volumetric flow rate (ml/min) at 5 x 1 cm i.d. 4.0
pH stability 2–10
Supplied as Bulk/slurry 50% in 25% ethanol
Storage 4°C, do not freeze

When a Thiophilic-Superflow Resin column was used to purify whole rabbit serum, albumin was effectively removed in the nonadsorbed fraction, allowing the elution of highly purified intact IgG (Figures 5 & 6). Thiophilic adsorbents can also purify other types of proteins such as horseradish peroxidase (Chaga et al. 1992), allergens (Goubran-Botros et al. 1998), glutathione peroxidase (Huang et al. 1994), procollagen (Pedersen & Bonde 1994), acetolactate synthase (Poulsen & Stougaard 1989), insect hemolymph proteins (Samaraweera et al. 1992), serpins (Rosenkrands et al. 1994), lactate dehydrogenase (Kminkova & Kucera 1998), and tuberculosis antigen proteins (Rosenkrands et al. 1998).

Figure 6. Analysis of purified IgG fractions.

Figure 6. Analysis of purified IgG fractions. Analytical size exclusion chromatography was performed on the purified fractions from Figure 5. Results indicate that the albumin, which constitutes 60–70% of the whole serum, is removed in the nonadsorbed fraction from whole rabbit serum (Panel A) and wash (Panel B). Then, the intact IgG from Peak II is eluted (Panel C).

References  

Chaga, G., Andersson, L., Ersson, B. & Berg, M. Use of immobilized metal ions as a negative adsorbent for purification of enzymes: Application to phosphoglycerate mutase from chicken muscle extract and horseradish peroxidase. Biomed. Chromatogr. 6, 172–176 (1992).

Goubran Botros, H., Rabillon, J., Grégoire, C., David, B. & Dandeu, J. P. Thiophilic adsorption chromatography: purification of Equ c2 and Equ c3, two horse allergens from horse sweat. J. Chromatogr. B. Biomed. Sci. Appl. 710, 57–65 (1998).

Hansen, P., Scoble, J. A., Hanson, B. & Hoogenraad, N. J. Isolation and purification of immunoglobulins from chicken eggs using thiophilic interaction chromatography. J. Immunol. Methods 215, 1–7 (1998).

Huang, K., Lauridsen, E. & Clausen, J. The uptake of Na-selenite in rat brain. Localization of new glutathione peroxidases in the rat brain. Biol. Trace Elem. Res. 46, 91–102

Hutchens, T. W. & Porath, J. Thiophilic adsorption of immunoglobulins--analysis of conditions optimal for selective immobilization and purification. Anal. Biochem. 159, 217–26 (1986).

Kminková, M. & Kucera, J. Purification of carp (Cyprinus carpio) hepatopancreatic lactate dehydrogenase (EC 1.1.1.27). Prep. Biochem. Biotechnol. 28, 313–7 (1998).

Lutomski, D., Joubert-Caron, R., Bourin, P., Bladier, D. & Caron, M. Use of thiophilic adsorption in the purification of biotinylated Fab fragments. J. Chromatogr. B. Biomed. Appl. 664, 79–82 (1995).

Oscarsson, S., Angulo-Tatis, D., Chaga, G. & Porath, J. Amphiphilic agarose-based adsorbents for chromatography comparative study of adsorption capacities and desorption efficiencies. J. Chromatogr. A 689, 3–12 (1995).

Oscarsson, S., Medin, A. & Porath, J. Kinetic and conformational factors involved in chemisorption and adsorption of proteins on mercaptopyridine-derivatized agarose. J. Colloid Interface Sci. 152, 114–124 (1992).

Pedersen, B. J. & Bonde, M. Purification of human procollagen type I carboxyl-terminal propeptide cleaved as in vivo from procollagen and used to calibrate a radioimmunoassay of the propeptide. Clin. Chem. 40, (1994).

Porath, J., Belew, M., Maisano, F. & Olin, B. Group specific protein adsorbents based on "thiophilic" interaction. In B. Rand (Ed.) Physical Chemistry of Colloids and Macromolecules, Uppsala, Sweden, 137–142 (1984).

Porath, J. Immobilized Metal Ion Affinity Chromatography-A powerful method for protein purification. In H. Tschelsche (Ed.), Modern Methods in Protein Chemistry, Berlin & NY: Walter de Gruyter & Co., 85–95(1985).

Poulsen, C. & Stougaard, P. Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli. Eur. J. Biochem. 185, 433–9 (1989).

Rosenkrands, I. et al. Identification and characterization of a 29-kilodalton protein from Mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. Infect. Immun. 66, 2728–35 (1998).

Rosenkrands, I., Hejgaard, J., Rasmussen, S. K. & Bjørn, S. E. Serpins from wheat grain. FEBS Lett. 343, 75–80 (1994).

Samaraweera, P., Porath, J. & Law, J. H. Separation of insect hemolymph proteins by cascade-mode multi-affinity chromatography. Arch. Insect Biochem. Physiol. 20, 243–51 (1992).

Schulze, R. A., Kontermann, R. E., Queitsch, I., Dübel, S. & Bautz, E. K. Thiophilic adsorption chromatography of recombinant single-chain antibody fragments. Anal. Biochem. 220, 212–4 (1994).

Scoble, J. A. & Scopes, R. K. Ligand structure of the divinylsulfone-based T-gel. J. Chromatogr. A 787, 47–54 (1997).

Yurov, G. K., Neugodova, G. L., Verkhovsky, O. A. & Naroditsky, B. S. Thiophilic adsorption: rapid purification of F(ab)2 and Fc fragments of IgG1 antibodies from murine ascitic fluid.J. Immunol. Methods 177, 29–33 (1994).

Related products

Cat. # Product Size License Quantity Details
635616 Thiophilic-Superflow Resin 10 mL USD $207.00

Thiophilic-Superflow Resin is an adsorbent for large-scale or FPLC purifcation of various proteins, including IgG, IgM, IgY, and IgE antibodies. Superflow allows for flow rates of as high as 5 ml/cm2 min.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Structure of Thiophilic Resin

Structure of Thiophilic Resin
Structure of Thiophilic Resin.

Back

Purification using Thiophilic Resin vs Protein A

Purification using Thiophilic Resin vs Protein A
Purification using Thiophilic Resin vs Protein A. Purification with Thiophilic Resin allows elution in any buffer with pH 7.0. In contrast, Protein A requires elution in a buffer with pH lower than 7.0. Protein A also requires a dialysis step, which can be omitted with Thiophilic Resin.

Back

Single-chain antibody purification with Thiophilic Resin

Single-chain antibody purification with Thiophilic Resin
Single-chain antibody purification with Thiophilic Resin. SDS-PAGE analysis of the following samples: bacterial lysate expressing scFv215 (Lane 2), periplasmic fraction (Lane 3), peak fraction from Ni-NTA (Lane 4), and peak fraction from Thiophilic Resin (Lane 5). (Schulze, R. A. et al. (1994) Anal. Biochem. 220(1):212–214. Permission to reprint obtained.).

Back

IgY purification from chicken egg using Thiophilic Resin

IgY purification from chicken egg using Thiophilic Resin
IgY purification from chicken egg using Thiophilic Resin. Panel A. SDS-PAGE analysis of fractions from purification of chicken egg immunoglobulins. Lanes 7 & 10 each contain 10 mg of protein, and all other lanes contain 25 mg each. Lane 1: egg yolk extract supernatant. Lane 2: Supernatant after 60% SAS (Saturated concentration of Ammonium Sulfate). Lane 3: Wash with 60% SAS. Lane 4: Pellet after 60% SAS. Lane 5: column load. Lane 6: unbound material. Lane 7: eluted material. Lanes 8–10: purification with another thiophilic resin. Panel B. Immunoblot using 1/10 the amount of material in Panel A. IgY was detected with polyclonal rabbit anti-chicken HRP-conjugate. M=molecular weight. (Hansen, P. et al. (1998). J. Immun. Methods 215(1–2):1–7. Permission to reprint obtained).

Back

635616: Thiophilic-Superflow Resin

635616: Thiophilic-Superflow Resin
635617 Thiophilic-Superflow Resin 100 mL USD $1609.00

Thiophilic-Superflow Resin is an adsorbent for large-scale or FPLC purifcation of various proteins, including IgG, IgM, IgY, and IgE antibodies. Superflow allows for flow rates of as high as 5 ml/cm2 min.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Structure of Thiophilic Resin

Structure of Thiophilic Resin
Structure of Thiophilic Resin.

Back

Purification using Thiophilic Resin vs Protein A

Purification using Thiophilic Resin vs Protein A
Purification using Thiophilic Resin vs Protein A. Purification with Thiophilic Resin allows elution in any buffer with pH 7.0. In contrast, Protein A requires elution in a buffer with pH lower than 7.0. Protein A also requires a dialysis step, which can be omitted with Thiophilic Resin.

Back

Single-chain antibody purification with Thiophilic Resin

Single-chain antibody purification with Thiophilic Resin
Single-chain antibody purification with Thiophilic Resin. SDS-PAGE analysis of the following samples: bacterial lysate expressing scFv215 (Lane 2), periplasmic fraction (Lane 3), peak fraction from Ni-NTA (Lane 4), and peak fraction from Thiophilic Resin (Lane 5). (Schulze, R. A. et al. (1994) Anal. Biochem. 220(1):212–214. Permission to reprint obtained.).

Back

IgY purification from chicken egg using Thiophilic Resin

IgY purification from chicken egg using Thiophilic Resin
IgY purification from chicken egg using Thiophilic Resin. Panel A. SDS-PAGE analysis of fractions from purification of chicken egg immunoglobulins. Lanes 7 & 10 each contain 10 mg of protein, and all other lanes contain 25 mg each. Lane 1: egg yolk extract supernatant. Lane 2: Supernatant after 60% SAS (Saturated concentration of Ammonium Sulfate). Lane 3: Wash with 60% SAS. Lane 4: Pellet after 60% SAS. Lane 5: column load. Lane 6: unbound material. Lane 7: eluted material. Lanes 8–10: purification with another thiophilic resin. Panel B. Immunoblot using 1/10 the amount of material in Panel A. IgY was detected with polyclonal rabbit anti-chicken HRP-conjugate. M=molecular weight. (Hansen, P. et al. (1998). J. Immun. Methods 215(1–2):1–7. Permission to reprint obtained).

Back

635617: Thiophilic-Superflow Resin

635617: Thiophilic-Superflow Resin

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2023 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • COVID-19 research
  • Next-generation sequencing
  • Diagnostic solutions
  • Real-time PCR
  • Stem cell research
  • mRNA and cDNA synthesis
  • PCR
  • Cloning
  • Nucleic acid purification
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • New products
  • Special offers
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
  • Reproductive health
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
  • Free samples
  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
  • GoStix Plus special offers
  • PCR samples
  • Services & Support
  • Instrument services
  • Stem cell services
  • Gene and cell therapy manufacturing
  • Customer service
  • Sales
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • Partnering & Licensing
  • Vector information
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Online tools
  • GoStix Plus FAQs
  • Vector information
  • Vector document overview
  • Vector document finder
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • cDNA synthesis
  • Real-time PCR
  • Nucleic acid purification
  • PCR
  • Cloning
  • Stem cell research
  • Gene function
  • Protein research
  • Antibodies and ELISA
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • APPLICATIONS
  • Molecular diagnostics
  • Vaccine development
  • Pathogen detection
  • Immunotherapy research
  • Cancer research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Interview: adapting to change with Takara Bio
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • ESM Collection Kit forms
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • SMART-Seq Pro Biomarker Discovery Contest
  • DNA extraction educational activity
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Company benefits
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us