His60 nickel resin beats Ni-NTA resin for his-tag purification

His60 Ni Superflow Resin is a high-capacity Ni-IDA resin for the efficient purification of recombinant his-tagged proteins from bacterial, mammalian, and baculovirus-infected cells. His60 Ni resin is compatible with batch/gravity-flow applications, as well as with the major automated liquid chromatography systems and manual syringe processing, and it outperforms Ni-NTA resins (in capacity and purity). The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. His60 Ni Superflow Resin is available in a variety of sizes and as prepacked His60 Ni Gravity Columns. Using the same resin for small- and large-scale purifications means that you do not need to re-optimize conditions when you scale-up. The purified recombinant his-tagged proteins can be used for protein labeling, immunization to produce antibodies, or in animal studies.

The purification of recombinant his-tagged proteins consists of cell lysis/extraction, binding, washing, and elution. His60 Ni resin allows one-step protein purification under either native or denaturing conditions. Purified recombinant his-tagged proteins can be eluted with imidazole, or by a reduction in pH. His60 Ni resin is compatible with batch/gravity-flow applications, as well as with the major automated liquid chromatography systems or manual syringe processing.

• Use His60 Ni resin to obtain better purification of His-tagged proteins than Ni-NTA resin can provide.
• Purify recombinant his-tagged proteins from any expression system, including baculovirus, mammalian cells, yeast, and bacteria.
• Use purified proteins for any of the following applications:
  • Protein labeling
  • Immunization to produce antibodies
  • Animal studies

• His60 Ni FPLC cartridges
  • Prefilled 1-ml and 5-ml cartridges for his-tagged protein purification on any LC system, such as ÄKTA or FPLC, or for manual purification using a syringe.
• TALON cobalt resin
  • TALON resin provides the very highest purity for his-tagged proteins when compared to any nickel resin.
• His-tag detection antibodies
  • Use highly sensitive antibodies to detect his-tagged recombinant proteins in Western blot, ELISA, and immunohistochemical assays.
• ProteoGuard protease inhibitor cocktail
  • Use this EDTA-free protease inhibitor cocktail to suppress proteolysis in all your cell lysates. We recommend using the ProteoGuard cocktail with all our protein purification products.