Mammalian expression overview

Learn how to choose the best mammalian expression system for your specific application from our selection of pCMV-based vector systems, which incorporate HA-, or Myc-fusion tags. All of these systems provide the following advantages:

Constitutive, high-level expression in a wide variety of mammalian cells
Efficient immunoprecipitation, affinity purification, Western blotting, and immunofluorescent staining
Minimal interference with protein function due to the small size of the tag
Correct protein folding, assembly, and incorporation of post-translational modifications needed for biological activity

Selection guide for mammalian expression systems
	Protein yield per volume of culture	Applications/advantages
pHEK293 Ultra Expression Vectors	see product overview	The pHEK293 Ultra Expression Vectors are plasmid vectors developed for transient overexpression of recombinant proteins in human HEK293 cells. Protein expression using these vectors is 2–10 times higher than what is obtained from vectors with conventional cytomegalovirus (CMV)-derived promoters Ideal for production of biomedically relevant proteins, including monoclonal antibodies Learn more »
HA-tag expression system	10 µg/ml–10 mg/ml	Enables immunoprecipitation, purification, and detection of HA-tagged proteins Includes a highly specific antibody (available separately), which does not cross-react with endogenous proteins Allows purification of HA-tagged proteins using a nickel-based IMAC resin Learn more »
Myc-tag expression system	10 µg/ml–10 mg/ml	Enables immunoprecipitation, purification, and detection of Myc-tagged proteins Includes a highly specific antibody (available separately), which does not cross-react with endogenous proteins Allows confirmation of yeast two-hybrid interactions in mammalian cells Learn more »

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