- Capturem membrane technology
- Mass spectrometry digestion reagents
- Why tag a protein?
- Tech note: cobalt resin
- Purification methods overview
- Simplified purification of active, secreted his-tagged proteins
- Overview: His60
- Tech note: Capturem technology
- Tech note: Capturem large volume
- Magnetic beads
- TALON resin selection guide
- Selection guide: His60 resin
- FAQs: TALON
- Antibody purification
- Other tag purification
- Phosphoprotein and glycoprotein purification
- Expression systems
High-purity his-tagged protein purification with TALON resin
TALON resin for his-tagged protein purification lets you prepare exceptionally pure his-tagged proteins from bacterial, mammalian, yeast, and baculovirus-infected cells, under native or denaturing conditions. TALON resin is an immobilized metal affinity chromatography (IMAC) resin charged with cobalt, which binds to his-tagged proteins with higher specificity than nickel-charged resins. As a result, TALON resin delivers his-tagged proteins of the highest purity. In addition, each cobalt ion is bound to the resin at four sites, resulting in low metal ion leakage. TALON resin is available in a variety of formats. Use the following tables to decide which one is best for your specific application.
|TALON Single Step Columns||HisTALON Superflow Cartridge||HisTALON Gravity Columns||TALON Spin Columns|
|Binding capacity (mg protein/ml resin)||5–10||5–20||5–20||4–8|
|Starting material||Bacterial culture||Clarified lysate||Clarified lysate||Clarified lysate|
|Maximum flow rate (ml/min)||N/A||
|Bead size (µm)||300–500||60–160||45–165||45–165|
|Bulk resin formats|
|TALON Metal Affinity Resin||TALON Superflow Metal Affinity Resin||TALON Magnetic Beads||TALON CellThru|
|Binding capacity (mg protein/ml resin)||5–15||5–18||2–15||5–10|
|Starting material||Clarified lysate||Clarified lysate||Clarified lysate||Unclarified lysate|
|Maximum flow rate (ml/min)||0.5||50||N/A||13|
|Bead size (µm)||45–165||60–160||20–75||300–500|
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