Calpain inhibitor: Calpastatin protease inhibitor
Calpastatin is an endogenous protease inhibitor that acts specifically on calpain (a calcium-dependent cysteine protease). It contains four repetitive calpain inhibition sequences spanning 120 to 140 amino acid residues (domains I, II, and IV) and an N-terminal non-homologous sequence (domain L). The product consists of highly purified recombinant human calpastatin domain I.
Calpain protease inhibition
Recombinant Human Calpastatin Domain I
Homogeneous on SDS-polyacrylamide gel electrophoresis. Greater than 90% pure as determined by amino acid composition analysis.
- Molecular weight: 14 kDa
- Peptide length: 137 aa
- Typical activity: 50 nM of calpastatin domain I completely inhibits the activity of 7.5 µg/ml calpain I. 15 nM of calpastatin domain I inhibits 50% of the activity of 7.5 µg/ml calpain I.
Lyophilized white powder
Asada, K. et al. cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins. J. Enz. Inhib. 3:49 (1989).
Kanaki, R. and Murachi, T. Physiological significance of the calpain-calpastatin system. Protein, Nucleic Acid and Enzyme 32:116 (Japanese Journal) (1987).
Uemori, T. et al. Characterization of a functional domain of human calpastatin. Biochem. Biophys. Res. Comm. 166:1485 (1990).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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