N-glycan degradation: Alpha 1,2-L Fucosidase

Alpha 1,2-L Fucosidase specifically cleaves the alpha 1,2 fucoside bond, liberating L-fucose from 2²-alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from porcine gastric mucin oligosaccharides. It also liberates L-fucose from N-acetyllactosamine-type bi-antennary sugar chains with alpha 1,6 fucoside bonds, but the rate of this reaction is extremely slow. Alpha 1,2-L Fucosidase can be used during N-glycan degradation.

Cat. #	Product	Size		License	Quantity	Details
4451	Alpha 1,2-L-Fucosidase	2 Units	USD $466.00
Intended usage: Specific cleavage of α1,2-L-fucoside bonds. This enzyme releases ^L-fucose from 22-α-^L-fucosyl lactose (Fucα1-2Galβ1-4Glc) and from porcine gastric mucin oligosaccharides. It also releases L-fucose from a biantennary N-acetyllactosamin type sugar chain with α1,6-fucoside bond (PA-Sugar Chain 009; Cat. #4109), but at a much lower reaction rate. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Substrate specificity of alpha 1,2-L-fucosidase (Cat.# 4451) Substrate specificity of alpha 1,2-L-fucosidase (Cat.# 4451). Back 4451: Alpha-1,2-L-Fucosidase

More Information

Applications

Cleavage of the alpha 1,2 fucoside bond during N-glycan degradation
Liberation of L-fucose from 2²-alpha-L-fucosyl lactose (Fuc alpha1-2 Gal beta1-4 Glc) and from porcine gastric mucin oligosaccharides

Source

Corynebacterium sp.

Storage

Under 4°C and dry conditions. A stock solution of 20 U/ml is stable for at least 3 months at 4°C. The stock solution may be frozen and thawed several times without loss of activity.

Properties

Systematic name: alpha-L-fucoside fucohydrolase (E.C. 3.2.1.51)
Molecular weight: 43 kDa (gel filtration)
K₂ (substrate): 4.7 x 10^-5 M (p-NP-fucose)
Inhibitors: Ag⁺, Hg²⁺, Cu²⁺
Optimum pH: pH 8.5 (37°C, 10 min)
Optimum temperature: 34°C (pH 8.5, 10 min)
Range of pH stability: pH 5.5–9.5 (25°C, 60 min)
Thermal stability: Less than or equal to 26°C (pH 8.0, 60 min)

Form

Lyophilized product (white non-crystallized powder) containing 50 mM potassium phosphate (pH 8.0), 0.5 mM EDTA, and 0.025% sodium azide

References

Sano, M., Hayakawa, K. & Kato, I. Purification and characterization of alpha-L-fucosidase from Streptomyces species. J. Biol. Chem. 267, 1522–7 (1992).

Definition of activity

One unit is defined as the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenyl alpha-L-fucoside (p-NP-Fucose) in 1 minute at 37°C at pH 8.5.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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