Western Blot Stripping Buffer
The Western BLoT Stripping Buffer is a solution for removing primary and secondary antibodies from probed Western blot membranes. Antibody removal with this buffer can occur under mild conditions (room temperature, 30 min incubation), minimizing loss of immobilized protein from the membrane. When using a PVDF membrane, the same membrane can be stripped and reprobed 2–5 times. After stripping, membranes can be re-probed, either with a different concentration of primary antibody or with an entirely different primary antibody.
Overview
- Efficient antibody removal from Western blot membranes with little loss of bound protein
- Fast, simple protocol—immerse blot in stripping buffer and incubate at room temperature for 30 min
More Information
Applications
- Stripping Western blots for reprobing
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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