Lenti-X Transduction Sponge overview
The Lenti-X Transduction Sponge is a novel technology that provides a microfluidic environment for lentivirus transduction in a dissolvable, easy‑to‑use alginate sponge. Within the pores of the sponge, the cells and virus are gently colocalized, thus increasing transduction efficiency and preserving cell viability. Any researcher can easily achieve successful transductions using the sponge without the need for complicated spinoculation protocols, harsh chemical enhancers, or expensive specialized microfluidics equipment.
Learn more about the Lenti‑X Transduction Sponge, including its fast, user-friendly workflow and compatibility with multiple cell types. Sign up to try a sample of one of our transduction sponges!
Simple transduction workflow using the Lenti‑X Transduction Sponge. Cells and virus are mixed prior to applying to the sponge. The transduction mixture is incubated for 1 hr, followed by the addition of media and overnight incubation for 16–24 hr. No spinoculation is required. The next day, healthy, transduced cells are released using an optimized Release Buffer that depolymerizes the alginate matrix. Cells are then ready for subsequent analysis or continued culture.
Get a sample pack of one of our Lenti-X sponges
Fill out the form below to receive a sample of the Lenti‑X Transduction Sponge or the Lenti‑X T‑Cell Transduction Sponge. A sales representative will be in contact with you to discuss your project and collect your mailing address.
Lenti-X Transduction Sponge app note
Explore how the Lenti-X Transduction Sponge gently transduces primary cells with greater efficacy than conventional protocols which use spinoculation or chemical enhancers.
Lenti-X Transduction Sponge tech note
Use a 3D alginate sponge to maximize lentiviral transduction efficiency.
Lenti-X Transduction Sponge compatible cell types
One of the advantages of Lenti-X Transduction Sponge is its versatility, allowing the same easy protocol to be applied to a wide range of cell types, including both commonly used cell lines and previously difficult-to-transduce primary cells.
Lenti-X Transduction Sponge
The Lenti-X Transduction Sponge facilitates rapid and efficient lentiviral transduction without spinoculation or the use of a chemical transduction enhancer.
Lenti-X T-Cell Transduction Sponge
The Lenti-X T-Cell Transduction Sponge facilitates rapid and efficient lentiviral transduction of T cells in the presence of antibodies and cytokine without spinoculation or the use of a chemical transduction enhancer.
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