Recombinant lentivirus learning center

We offer a broad range of learning resources for lentiviral transduction to help your research. Watch videos, learn tips for improving viral transduction, and find products such as Lenti-X Concentrator and Lenti-X GoStix to make your experiments faster and easier. For recombinant retrovirus products, visit the Retro-X learning center.


Get a lentiviral workflow infographic poster.


Learn more about our lentiviral packaging mixes

Lentivirus clickable workflow brochure

Use our clickable workflow for tips and tools for every step of your lentiviral gene delivery workflow, from vector selection and cloning, to virus packaging and production, harvesting and titration, concentration and/or purification, and transduction.

Explore clickable workflow

This video introduces the GoStix Plus app, which is used with Lenti-X GoStix Plus lateral flow assays to quantify lentiviral p24 from packaging cell supernatants. After applying a small amount of sample to a GoStix cassette and waiting 10 minutes for the test to develop, simply scan the cassette with a smartphone running the app. The app also includes features for sharing results and providing feedback.

Lenti-X GoStix Plus product page


Lenti-X Sponge data

Explore how the Lenti-X Transduction Sponge gently transduces primary cells with greater efficacy than conventional protocols which use spinoculation or chemical enhancers.

Discover how the T-cell sponge—embedded with activation agents—effectively activates and transduces isolated T cells or PBMCs, while maintaining high cell viability

Learn how a 3D alginate sponge maximizes lentiviral transduction efficiency.


Selection guides

Product selection guide for lentiviral products including vector systems, titration kits, and other tools.

Highly optimized lentiviral vectors and expression systems for many applications.

Alternative methods to accurately measure lentivirus titer including p24 ELISA, qRT-PCR, and GoStix.

Use the workflow depicted on this page to find products, learning resources, and technical information.


Lentiviral packaging

Easily produce high-quality lentiviral particles from any SARS-CoV-2 spike variant mutant.

Learn about our Lenti-X fourth-generation packaging systems, optimized for viral yield, ease of use, and safety.

Obtain high titers in 96-well plates without optimizing or aliquoting lentiviral packaging reagents.

Learn about lentiviral vector safety information and guidelines.


Lentiviral tools and tips

The Lenti-X Transduction Sponge is a novel technology that provides a microfluidic environment for lentivirus transduction in a small, easy-to-use alginate sponge.

Calculate the IFU/ml for unknown samples from a known reference sample and the p24 titer values resulting from the Lenti-X p24 Rapid Titer Kit (Single Wash).

Just mix with your lentiviral supernatant, and spin in a standard centrifuge.

Alternative methods to accurately measure lentivirus titer including p24 ELISA, qRT-PCR, and GoStix.

Discussion of critical factors for successful lentiviral transduction

Watch these videos to learn how to use our Lenti-X GoStix Plus cassettes and companion app.

Tips for successful lentiviral titration using our simple, 10-minute assay.


Premade lentiviral particles

Overview of premade, highly concentrated and purified lentiviral particles.

Lentiviral particles for expression of fluorescent proteins.

Frequently asked questions involving our ready-made lentiviral particles.

Webinar series: lentiviral gene delivery made easy.


Learn more about our lentiviral packaging mixes

Video—fourth-generation lentiviral packaging

Generate lentiviral titers that far exceed most other commercially available packaging systems—you can expect titers of 107–108 infectious units (IFU) per ml, far higher than other popular systems generate. Watch the video in the first tab to find out how our fourth-generation packaging system can generate such high titers.

Learn about Lenti-X packaging Lentiviral workflow