Retroviral product selection guide
Use the list below to select the right retroviral product for your research. Choose from a wide variety of retroviral vectors, as well as retroviral packaging systems and cell lines, products for titrating and concentrating retrovirus, and retroviral transduction enhancers.
Retroviral vectors
Constitutive expression vectors
Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. Designed for high retroviral titers and high expression of your transgene; compatible with all of our retroviral packaging cell lines.
Tetracycline-inducible expression vectors (Tet-On 3G)
Obtain the tightest possible control of gene expression, using Tet-On 3G or Tet-Express systems.
Introduce and express target genes in pluripotent cell lines including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells.
Retroviral packaging systems and cell lines
GP2-293 Packaging Cell Line
GP2-293 stably expresses the essential viral packaging genes gag and pol. However, the viral envelope (env) must be supplied in trans via transient transfection. This results in higher titers and the flexibility to choose between envelopes. This cell line is not sold separately, but provided with both of our retroviral packaging systems:
Retro-X Universal Packaging System
The Retro-X Universal Packaging System includes GP2-293 cells and four different envelope plasmids, so you vary virus tropism (pantropic [VSV-G], amphotropic, ecotropic, or dualtropic) to suit your target cells.
Pantropic Retroviral Expression System
The Pantropic Retroviral Expression System includes our GP2-293 packaging cell line, the pVSV-G envelope plasmid, and two MMLV-based retroviral expression vectors that allow you to express your gene of interest from alternative promoters.
Alternatively, we offer the PT67 cell line that stably expresses gag, pol, and env (10A1-derived) essential packaging genes. This cell line can be purchased separately.
RetroPack PT67 Cell Line
Use the RetroPack PT67 Cell Line to create a stable cell line for continuous retrovirus production.
Titration kit
qRT-PCR titration kit
Simply purify viral RNA, and then quantify the number of retroviral genome copies using qRT-PCR and TB Green technologies.
Concentration reagent
Retro-X Concentrator
Increase retrovirus titer 100X without ultracentrifugation. Just mix your MMLV- or MSCV-based retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
Transduction enhancers
Magnetic transduction beads
Perform retroviral transduction in only 30 min using the magnetic beads supplied with Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull retrovirus (or lentivirus) to your cells with a magnet.
Coat your culture dish with this multivalent molecule to significantly increase transduction efficiency. RetroNectin colocalizes retrovirus (or lentivirus) particles with your target cell. Perfect for hard-to-transduce suspension cells and stem cells.
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