Retroviral product selection guide
Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. Designed for high retroviral titers and high expression of your transgene; compatible with all of our retroviral packaging cell lines.
Obtain the tightest possible control of gene expression, using Tet-On 3G or Tet-Express systems.
Retroviral packaging systems and cell lines
GP2-293 Packaging Cell Line
GP2-293 stably expresses the essential viral packaging genes gag and pol. However, the viral envelope (env) must be supplied in trans via transient transfection. This results in higher titers and the flexibility to choose between envelopes. This cell line is not sold separately, but provided with both of our retroviral packaging systems:
The Retro-X Universal Packaging System includes GP2-293 cells and four different envelope plasmids, so you vary virus tropism (pantropic [VSV-G], amphotropic, ecotropic, or dualtropic) to suit your target cells.
Alternatively, we offer the PT67 cell line that stably expresses gag, pol, and env (10A1-derived) essential packaging genes. This cell line can be purchased separately.
Use the RetroPack PT67 Cell Line to create a stable cell line for continuous retrovirus production.
Simply purify viral RNA, and then quantify the number of retroviral genome copies using qRT-PCR and TB Green technologies.
Increase retrovirus titer 100X without ultracentrifugation. Just mix your MMLV- or MSCV-based retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
Perform retroviral transduction in only 30 min using the magnetic beads supplied with Lenti-X Accelerator. Simply bind your viral supernatant to the beads and pull retrovirus (or lentivirus) to your cells with a magnet.
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