The Adeno-X Adenoviral System 3 (Tet-On 3G Inducible) from Takara Bio combines the tightest and most sensitive control of gene expression with the most advanced commercially available adenoviral vector system. With this system, tightly-controlled inducible expression is as easy as constitutive expression, and cloning into an adenoviral vector is as straightforward as cloning into any plasmid.
How does the Tet-On 3G inducible system work?
Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox), a tetracycline analog. When bound by Dox, the Tet-On 3G protein undergoes a conformational change that allows it to bind to tet operator (tetO) sequences located in PTRE3G (Figure 1). In contrast to TetR-based systems, Tet-On technologies activate rather than repress transcription, a critical difference which results in far lower basal expression, higher maximal expression, a more rapid response time—and ultimately, makes them the first choice for conditional expression.